2nw0

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(New page: 200px<br /><applet load="2nw0" size="350" color="white" frame="true" align="right" spinBox="true" caption="2nw0, resolution 1.600&Aring;" /> '''Crystal structure o...)
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[[Image:2nw0.gif|left|200px]]<br /><applet load="2nw0" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2nw0, resolution 1.600&Aring;" />
 
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'''Crystal structure of a lysin'''<br />
 
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==Overview==
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==Crystal structure of a lysin==
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Lysins are peptidoglycan hydrolases that are produced by bacteriophage and, act to lyse the bacterial host cell wall during progeny phage release., Here, we describe the structure and function of a novel, bacteriophage-derived lysin, PlyB, which displays potent lytic activity, against the Bacillus anthracis-like strain ATCC 4342. This molecule, comprises an N-terminal catalytic domain (PlyB(cat)) and a C-terminal, bacterial SH3-like domain, SH3b. It is shown that both domains are, required for effective catalytic activity against ATCC 4342. Further, PlyB, has specific activity comparable to the phage lysin PlyG, an amidase being, developed as a therapeutic against anthrax. In contrast to PlyG, however, the 1.6 A X-ray crystal structure of PlyB(cat) reveals that the catalytic, domain adopts the glycosyl hydrolase (GH)-25, rather than phage T7, lysozyme-like fold. PlyB therefore represents a new class of anthrax lysin, and a new defensive tool in the armament against anthrax-mediated, bioterrorism.
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<StructureSection load='2nw0' size='340' side='right'caption='[[2nw0]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2nw0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacteriophage_sp. Bacteriophage sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NW0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NW0 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nw0 OCA], [https://pdbe.org/2nw0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nw0 RCSB], [https://www.ebi.ac.uk/pdbsum/2nw0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nw0 ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nw/2nw0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2nw0 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Lysins are peptidoglycan hydrolases that are produced by bacteriophage and act to lyse the bacterial host cell wall during progeny phage release. Here, we describe the structure and function of a novel bacteriophage-derived lysin, PlyB, which displays potent lytic activity against the Bacillus anthracis-like strain ATCC 4342. This molecule comprises an N-terminal catalytic domain (PlyB(cat)) and a C-terminal bacterial SH3-like domain, SH3b. It is shown that both domains are required for effective catalytic activity against ATCC 4342. Further, PlyB has specific activity comparable to the phage lysin PlyG, an amidase being developed as a therapeutic against anthrax. In contrast to PlyG, however, the 1.6 A X-ray crystal structure of PlyB(cat) reveals that the catalytic domain adopts the glycosyl hydrolase (GH)-25, rather than phage T7 lysozyme-like fold. PlyB therefore represents a new class of anthrax lysin and a new defensive tool in the armament against anthrax-mediated bioterrorism.
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==About this Structure==
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The 1.6 A crystal structure of the catalytic domain of PlyB, a bacteriophage lysin active against Bacillus anthracis.,Porter CJ, Schuch R, Pelzek AJ, Buckle AM, McGowan S, Wilce MC, Rossjohn J, Russell R, Nelson D, Fischetti VA, Whisstock JC J Mol Biol. 2007 Feb 16;366(2):540-50. Epub 2006 Nov 18. PMID:17182056<ref>PMID:17182056</ref>
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2NW0 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Unidentified_phage Unidentified phage] with <scene name='pdbligand=ACT:'>ACT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NW0 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The 1.6 A crystal structure of the catalytic domain of PlyB, a bacteriophage lysin active against Bacillus anthracis., Porter CJ, Schuch R, Pelzek AJ, Buckle AM, McGowan S, Wilce MC, Rossjohn J, Russell R, Nelson D, Fischetti VA, Whisstock JC, J Mol Biol. 2007 Feb 16;366(2):540-50. Epub 2006 Nov 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17182056 17182056]
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</div>
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[[Category: Lysozyme]]
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<div class="pdbe-citations 2nw0" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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[[Category: Unidentified phage]]
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[[Category: Buckle, A.M.]]
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[[Category: Porter, C.J.]]
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[[Category: Whisstock, J.C.]]
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[[Category: ACT]]
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[[Category: beta barrel]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:00:30 2008''
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==See Also==
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*[[Lysin 3D structures|Lysin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Bacteriophage sp]]
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[[Category: Large Structures]]
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[[Category: Buckle AM]]
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[[Category: Porter CJ]]
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[[Category: Whisstock JC]]

Current revision

Crystal structure of a lysin

PDB ID 2nw0

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