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2nx9

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(New page: 200px<br /><applet load="2nx9" size="350" color="white" frame="true" align="right" spinBox="true" caption="2nx9, resolution 1.70&Aring;" /> '''Crystal structure of...)
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[[Image:2nx9.jpg|left|200px]]<br /><applet load="2nx9" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2nx9, resolution 1.70&Aring;" />
 
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'''Crystal structure of the carboxyltransferase domain of the oxaloacetate decarboxylase Na+ pump from Vibrio cholerae'''<br />
 
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==Overview==
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==Crystal structure of the carboxyltransferase domain of the oxaloacetate decarboxylase Na+ pump from Vibrio cholerae==
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Oxaloacetate decarboxylase is a membrane-bound multiprotein complex that, couples oxaloacetate decarboxylation to sodium ion transport across the, membrane. The initial reaction catalyzed by this enzyme machinery is the, carboxyl transfer from oxaloacetate to the prosthetic biotin group. The, crystal structure of the carboxyltransferase at 1.7 A resolution shows a, dimer of alpha(8)beta(8) barrels with an active site metal ion, identified, spectroscopically as Zn(2+), at the bottom of a deep cleft. The enzyme is, completely inactivated by specific mutagenesis of Asp17, His207 and, His209, which serve as ligands for the Zn(2+) metal ion, or by Lys178 near, the active site, suggesting that Zn(2+) as well as Lys178 are essential, for the catalysis. In the present structure this lysine residue is, hydrogen-bonded to Cys148. A potential role of Lys178 as initial acceptor, of the carboxyl group from oxaloacetate is discussed.
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<StructureSection load='2nx9' size='340' side='right'caption='[[2nx9]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2nx9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NX9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NX9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nx9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nx9 OCA], [https://pdbe.org/2nx9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nx9 RCSB], [https://www.ebi.ac.uk/pdbsum/2nx9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nx9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q6A1F6_VIBCL Q6A1F6_VIBCL]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nx/2nx9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2nx9 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Oxaloacetate decarboxylase is a membrane-bound multiprotein complex that couples oxaloacetate decarboxylation to sodium ion transport across the membrane. The initial reaction catalyzed by this enzyme machinery is the carboxyl transfer from oxaloacetate to the prosthetic biotin group. The crystal structure of the carboxyltransferase at 1.7 A resolution shows a dimer of alpha(8)beta(8) barrels with an active site metal ion, identified spectroscopically as Zn(2+), at the bottom of a deep cleft. The enzyme is completely inactivated by specific mutagenesis of Asp17, His207 and His209, which serve as ligands for the Zn(2+) metal ion, or by Lys178 near the active site, suggesting that Zn(2+) as well as Lys178 are essential for the catalysis. In the present structure this lysine residue is hydrogen-bonded to Cys148. A potential role of Lys178 as initial acceptor of the carboxyl group from oxaloacetate is discussed.
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==About this Structure==
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Crystal structure of the carboxyltransferase domain of the oxaloacetate decarboxylase Na+ pump from Vibrio cholerae.,Studer R, Dahinden P, Wang WW, Auchli Y, Li XD, Dimroth P J Mol Biol. 2007 Mar 23;367(2):547-57. Epub 2006 Dec 19. PMID:17270211<ref>PMID:17270211</ref>
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2NX9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Oxaloacetate_decarboxylase Oxaloacetate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.3 4.1.1.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NX9 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal Structure of the Carboxyltransferase Domain of the Oxaloacetate Decarboxylase Na(+) Pump from Vibrio cholerae., Studer R, Dahinden P, Wang WW, Auchli Y, Li XD, Dimroth P, J Mol Biol. 2007 Mar 23;367(2):547-57. Epub 2006 Dec 19. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17270211 17270211]
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</div>
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[[Category: Oxaloacetate decarboxylase]]
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<div class="pdbe-citations 2nx9" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Vibrio cholerae]]
[[Category: Vibrio cholerae]]
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[[Category: Dimroth, P.]]
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[[Category: Dimroth P]]
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[[Category: Studer, R.]]
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[[Category: Studer R]]
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[[Category: ZN]]
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[[Category: biotin enzymes]]
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[[Category: carboxyltransferase structure]]
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[[Category: oxaloacetate decarboxylase]]
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[[Category: tim-barrel fold]]
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[[Category: zn2+ binding site]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:01:22 2008''
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Current revision

Crystal structure of the carboxyltransferase domain of the oxaloacetate decarboxylase Na+ pump from Vibrio cholerae

PDB ID 2nx9

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