3ako

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[[Image:3ako.jpg|left|200px]]
 
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==Crystal Structure of the Reassembled Venus==
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The line below this paragraph, containing "STRUCTURE_3ako", creates the "Structure Box" on the page.
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<StructureSection load='3ako' size='340' side='right'caption='[[3ako]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ako]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Plant_transformation_vector_pSITEII-4C1 Plant transformation vector pSITEII-4C1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AKO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AKO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CR2:{(4Z)-2-(AMINOMETHYL)-4-[(4-HYDROXYPHENYL)METHYLIDENE]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CR2</scene>, <scene name='pdbligand=PE8:3,6,9,12,15,18,21-HEPTAOXATRICOSANE-1,23-DIOL'>PE8</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3ako| PDB=3ako | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ako FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ako OCA], [https://pdbe.org/3ako PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ako RCSB], [https://www.ebi.ac.uk/pdbsum/3ako PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ako ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Bimolecular fluorescence complementation (BiFC) assay has been used widely to visualize protein-protein interactions in cells. However, there is a problem that fluorescent protein fragments have an ability to associate with each other independent of an interaction between proteins fused to the fragments. To facilitate the BiFC assay, we have attempted to determine the structure and characteristics of reassembled fluorescent protein, Venus. The anion-exchange chromatography showed an oligomer and a monomer of reassembled Venus. Our results suggested that the oligomer was formed by beta-strands swapping without any serious steric clashes and was converted to the monomer. Crystal structure of reassembled Venus had an 11-stranded beta-barrel fold, typical of GFP-derived fluorescent proteins. Based on the structural features, we have mutated to beta-strand 7 and measured T(m) values. The results have revealed that the mutation influences the thermal stability of reassembled fluorescent complex.
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===Crystal Structure of the Reassembled Venus===
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Structure and characteristics of reassembled fluorescent protein, a new insight into the reassembly mechanisms.,Isogai M, Kawamoto Y, Inahata K, Fukada H, Sugimoto K, Tada T Bioorg Med Chem Lett. 2011 May 15;21(10):3021-4. Epub 2011 Mar 16. PMID:21463942<ref>PMID:21463942</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ako" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 21463942 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21463942}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[3ako]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Other_sequences Other sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AKO OCA].
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[[Category: Plant transformation vector pSITEII-4C1]]
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[[Category: Isogai M]]
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==Reference==
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[[Category: Tada T]]
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<ref group="xtra">PMID:021463942</ref><references group="xtra"/>
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[[Category: Other sequences]]
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[[Category: Isogai, M.]]
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[[Category: Tada, T.]]
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[[Category: Fluorescent protein]]
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[[Category: Gfp]]
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[[Category: Venus]]
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Current revision

Crystal Structure of the Reassembled Venus

PDB ID 3ako

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