2obm

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(New page: 200px<br /><applet load="2obm" size="350" color="white" frame="true" align="right" spinBox="true" caption="2obm, resolution 2.25&Aring;" /> '''Structural and bioch...)
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[[Image:2obm.gif|left|200px]]<br /><applet load="2obm" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2obm, resolution 2.25&Aring;" />
 
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'''Structural and biochemical analysis of a prototypical ATPase from the type III secretion system of pathogenic bacteria'''<br />
 
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==Overview==
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==Structural and biochemical analysis of a prototypical ATPase from the type III secretion system of pathogenic bacteria==
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The type III secretion system (T3SS) ATPase is the conserved and essential, inner-membrane component involved in the initial stages of selective, secretion of specialized T3SS virulence effector proteins from the, bacterial cytoplasm through to the infected host cell, a process crucial, to subsequent pathogenicity. Here we present the 1.8-A-resolution crystal, structure of the catalytic domain of the prototypical T3SS ATPase EscN, from enteropathogenic Escherichia coli (EPEC). Along with in vitro and in, vivo mutational analysis, our data show that the T3SS ATPases share, similarity with the F1 ATPases but have important structural and sequence, differences that dictate their unique secretory role. We also show that, T3SS ATPase activity is dependent on EscN oligomerization and describe the, molecular features and possible functional implications of a hexameric, ring model.
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<StructureSection load='2obm' size='340' side='right'caption='[[2obm]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2obm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O127:H6_str._E2348/69 Escherichia coli O127:H6 str. E2348/69]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OBM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OBM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2obm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2obm OCA], [https://pdbe.org/2obm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2obm RCSB], [https://www.ebi.ac.uk/pdbsum/2obm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2obm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/B7UMA6_ECO27 B7UMA6_ECO27]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ob/2obm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2obm ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The type III secretion system (T3SS) ATPase is the conserved and essential inner-membrane component involved in the initial stages of selective secretion of specialized T3SS virulence effector proteins from the bacterial cytoplasm through to the infected host cell, a process crucial to subsequent pathogenicity. Here we present the 1.8-A-resolution crystal structure of the catalytic domain of the prototypical T3SS ATPase EscN from enteropathogenic Escherichia coli (EPEC). Along with in vitro and in vivo mutational analysis, our data show that the T3SS ATPases share similarity with the F1 ATPases but have important structural and sequence differences that dictate their unique secretory role. We also show that T3SS ATPase activity is dependent on EscN oligomerization and describe the molecular features and possible functional implications of a hexameric ring model.
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==About this Structure==
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Structural analysis of a prototypical ATPase from the type III secretion system.,Zarivach R, Vuckovic M, Deng W, Finlay BB, Strynadka NC Nat Struct Mol Biol. 2007 Feb;14(2):131-7. Epub 2007 Jan 21. PMID:17237797<ref>PMID:17237797</ref>
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2OBM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=CA:'>CA</scene> and <scene name='pdbligand=ADP:'>ADP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OBM OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural analysis of a prototypical ATPase from the type III secretion system., Zarivach R, Vuckovic M, Deng W, Finlay BB, Strynadka NC, Nat Struct Mol Biol. 2007 Feb;14(2):131-7. Epub 2007 Jan 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17237797 17237797]
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</div>
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[[Category: Escherichia coli]]
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<div class="pdbe-citations 2obm" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Deng, W.]]
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<references/>
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[[Category: Finlay, B.B.]]
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__TOC__
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[[Category: Strynadka, N.C.J.]]
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</StructureSection>
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[[Category: Vuckovic, M.]]
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[[Category: Escherichia coli O127:H6 str. E2348/69]]
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[[Category: Zarivach, R.]]
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[[Category: Large Structures]]
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[[Category: ADP]]
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[[Category: Deng W]]
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[[Category: CA]]
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[[Category: Finlay BB]]
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[[Category: atpase]]
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[[Category: Strynadka NCJ]]
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[[Category: Vuckovic M]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:08:38 2008''
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[[Category: Zarivach R]]

Current revision

Structural and biochemical analysis of a prototypical ATPase from the type III secretion system of pathogenic bacteria

PDB ID 2obm

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