2xky

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (18:15, 18 October 2023) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2xky.png|left|200px]]
 
-
<!--
+
==Single particle analysis of Kir2.1NC_4 in negative stain==
-
The line below this paragraph, containing "STRUCTURE_2xky", creates the "Structure Box" on the page.
+
<SX load='2xky' size='340' side='right' viewer='molstar' caption='[[2xky]], [[Resolution|resolution]] 17.20&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2xky]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2XKY FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy , Hybrid , X-ray solution scattering, [[Resolution|Resolution]] 17.2&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2xky FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2xky OCA], [https://pdbe.org/2xky PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2xky RCSB], [https://www.ebi.ac.uk/pdbsum/2xky PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2xky ProSAT]</span></td></tr>
-
{{STRUCTURE_2xky| PDB=2xky | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/KCNJ2_MOUSE KCNJ2_MOUSE] Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium.
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The interaction of the extra-membranous domain of tetrameric inwardly rectifying Kir2.1 ion channels (Kir2.1NC(4)) with the membrane associated guanylate kinase protein PSD-95 has been studied using Transmission Electron Microscopy in negative stain. Three types of complexes were observed in electron micrographs corresponding to a 1:1 complex, a large self-enclosed tetrad complex and extended chains of linked channel domains. Using models derived from small angle X-ray scattering experiments in which high resolution structures from X-ray crystallographic and Nuclear Magnetic Resonance studies are positioned, the envelopes from single particle analysis can be resolved as a Kir2.1NC(4):PSD-95 complex and a tetrad of this unit (Kir2.1NC(4):PSD-95)(4). The tetrad complex shows the close association of the Kir2.1 cytoplasmic domains and the influence of PSD-95 mediated self-assembly on the clustering of these channels.
-
===SINGLE PARTICLE ANALYSIS OF KIR2.1NC_4 IN NEGATIVE STAIN===
+
Self-directed assembly and clustering of the cytoplasmic domains of inwardly rectifying Kir2.1 potassium channels on association with PSD-95.,Fomina S, Howard TD, Sleator OK, Golovanova M, O'Ryan L, Leyland ML, Grossmann JG, Collins RF, Prince SM Biochim Biophys Acta. 2011 Jul 5;1808(10):2374-2389. PMID:21756874<ref>PMID:21756874</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2xky" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_21756874}}, adds the Publication Abstract to the page
+
*[[Potassium channel 3D structures|Potassium channel 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 21756874 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_21756874}}
+
__TOC__
-
 
+
</SX>
-
==About this Structure==
+
[[Category: Large Structures]]
-
[[2xky]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2XKY OCA].
+
-
 
+
-
==Reference==
+
-
<ref group="xtra">PMID:021756874</ref><ref group="xtra">PMID:015723059</ref><references group="xtra"/>
+
[[Category: Mus musculus]]
[[Category: Mus musculus]]
-
[[Category: Collins, R F.]]
+
[[Category: Collins RF]]
-
[[Category: Fomina, S.]]
+
[[Category: Fomina S]]
-
[[Category: Golovanova, M.]]
+
[[Category: Golovanova M]]
-
[[Category: Grossmann, J G.]]
+
[[Category: Grossmann JG]]
-
[[Category: Howard, T D.]]
+
[[Category: Howard TD]]
-
[[Category: Leyland, M L.]]
+
[[Category: Leyland ML]]
-
[[Category: Prince, S M.]]
+
[[Category: O'Ryan L]]
-
[[Category: Ryan, L O.]]
+
[[Category: Prince SM]]
-
[[Category: Sleator, O K.]]
+
[[Category: Sleator OK]]
-
[[Category: Ion channel]]
+
-
[[Category: Membrane protein]]
+
-
[[Category: Metal transport]]
+

Current revision

Single particle analysis of Kir2.1NC_4 in negative stain

2xky, resolution 17.20Å

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2xky"
Personal tools