3paz

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[[Image:3paz.gif|left|200px]]<br />
 
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<applet load="3paz" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="3paz, resolution 1.73&Aring;" />
 
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'''REDUCED NATIVE PSEUDOAZURIN FROM A. FAECALIS'''<br />
 
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==Overview==
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==REDUCED NATIVE PSEUDOAZURIN FROM A. FAECALIS==
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In order to understand the origins of differences in redox potentials, among cupredoxins (small blue type I copper-containing proteins that, reversibly change oxidation state and interact with redox partners), we, have determined the structures of the native and two mutants (P80A and, P80I) of pseudoazurin from Alcaligenes faecalis S-6 in oxidized and, reduced forms at resolutions of 2.2 A in the worst case and 1.6 A in the, best case. The P80A mutation creates a surface pocket filled by a new, water molecule, whereas the P80I mutant excludes this water. Distinct, patterns of change occur in response to reduction for all three molecules:, the copper position shifts, Met 7 and Pro 35 move, and the relative, orientations of residues 81 to 16, 18 to the amide planes of 77 and 86, all change. ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9341204 (full description)]]
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<StructureSection load='3paz' size='340' side='right'caption='[[3paz]], [[Resolution|resolution]] 1.73&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3paz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PAZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PAZ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.73&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3paz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3paz OCA], [https://pdbe.org/3paz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3paz RCSB], [https://www.ebi.ac.uk/pdbsum/3paz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3paz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AZUP_ALCFA AZUP_ALCFA] This soluble electron transfer copper protein is required for the inactivation of copper-containing nitrite reductase in the presence of oxygen. Serves as a direct electron donor to the nitrite reductase.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pa/3paz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3paz ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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3PAZ is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]] with CU as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: CU. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=3PAZ OCA]].
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*[[Pseudoazurin|Pseudoazurin]]
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__TOC__
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==Reference==
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</StructureSection>
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Site-directed mutants of pseudoazurin: explanation of increased redox potentials from X-ray structures and from calculation of redox potential differences., Libeu CA, Kukimoto M, Nishiyama M, Horinouchi S, Adman ET, Biochemistry. 1997 Oct 28;36(43):13160-79. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9341204 9341204]
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[[Category: Alcaligenes faecalis]]
[[Category: Alcaligenes faecalis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Adman, E.T.]]
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[[Category: Adman ET]]
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[[Category: Libeu, C.A.P.]]
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[[Category: Libeu CAP]]
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[[Category: CU]]
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[[Category: cuproprotein]]
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[[Category: electron transfer]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:20:44 2007''
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Current revision

REDUCED NATIVE PSEUDOAZURIN FROM A. FAECALIS

PDB ID 3paz

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