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311d

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(New page: 200px<br /><applet load="311d" size="350" color="white" frame="true" align="right" spinBox="true" caption="311d, resolution 2.200&Aring;" /> '''THE ROLE OF HYDROGE...)
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[[Image:311d.gif|left|200px]]<br /><applet load="311d" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="311d, resolution 2.200&Aring;" />
 
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'''THE ROLE OF HYDROGEN BONDING IN MINOR-GROOVE DRUG-DNA RECOGNITION. STRUCTURE OF A BIS-AMIDINIUM DERIVATIVE OF HOECHST 33258 COMPLEXED TO THE DODECANUCLEOTIDE D(CGCGAATTCGCG)2'''<br />
 
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==Overview==
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==THE ROLE OF HYDROGEN BONDING IN MINOR-GROOVE DRUG-DNA RECOGNITION. STRUCTURE OF A BIS-AMIDINIUM DERIVATIVE OF HOECHST 33258 COMPLEXED TO THE DODECANUCLEOTIDE D(CGCGAATTCGCG)2==
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The crystal structure is reported of a complex between the, dodecanucleotide sequence d(CGCGAATTCGCG)2and an analogue of the DNA, binding drug Hoechst 33258, in which the piperazine ring has been replaced, by an amidinium group and the phenol ring by a phenylamidinium group. The, structure has been refined to an R factor of 19.5% at 2.2 A resolution., The drug is held in the minor groove by five strong hydrogen bonds, together with bridging water molecules at both ends. There are few other, contacts with the floor of the groove, indicating a lack of isohelicity, with the groove and suggesting (i) that the observed high DNA affinity of, this drug is primarily due to the array of hydrogen bonds and (ii) that, these more than compensate for its poor isohelicity.
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<StructureSection load='311d' size='340' side='right'caption='[[311d]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[311d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=311D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=311D FirstGlance]. <br>
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311D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=HT2:'>HT2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=311D OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HT2:5-AMIDINO-2-[2-(4-AMIDINOPHENYL)-5-BENZIMIDAZOLYL]BENZIMIDAZOLE'>HT2</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=311d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=311d OCA], [https://pdbe.org/311d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=311d RCSB], [https://www.ebi.ac.uk/pdbsum/311d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=311d ProSAT]</span></td></tr>
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Structure of a bis-amidinium derivative of hoechst 33258 complexed to dodecanucleotide d(CGCGAATTCGCG)2: the role of hydrogen bonding in minor groove drug-DNA recognition., Clark GR, Boykin DW, Czarny A, Neidle S, Nucleic Acids Res. 1997 Apr 15;25(8):1510-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9162901 9162901]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Boykin, D.W.]]
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</StructureSection>
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[[Category: Clark, G.R.]]
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[[Category: Large Structures]]
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[[Category: Czarny, A.]]
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[[Category: Boykin DW]]
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[[Category: Neidle, S.]]
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[[Category: Clark GR]]
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[[Category: HT2]]
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[[Category: Czarny A]]
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[[Category: b-dna]]
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[[Category: Neidle S]]
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[[Category: complexed with drug]]
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[[Category: double helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:34:45 2008''
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Current revision

THE ROLE OF HYDROGEN BONDING IN MINOR-GROOVE DRUG-DNA RECOGNITION. STRUCTURE OF A BIS-AMIDINIUM DERIVATIVE OF HOECHST 33258 COMPLEXED TO THE DODECANUCLEOTIDE D(CGCGAATTCGCG)2

PDB ID 311d

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