313d

[[Image:313d.gif|left|200px]]<br /><applet load="313d" size="350" color="white" frame="true" align="right" spinBox="true"

caption="313d, resolution 1.680&Aring;" />

'''Z-DNA HEXAMER WITH 5' OVERHANGS THAT FORM A REVERSE HOOGSTEEN BASE PAIR'''<br />

==Overview==

We have solved the structures of the homoduplex d(Gm5CGCGCG)2, and the, heteroduplexes d(GCGCGCG)/d(TCGCGCG) and d(GCGCGCG)/d(CCGCGCG). The, structures form six base-pairs of identical Z-DNA duplexes with single, nucleotides overhanging at the 5'-ends. The overhanging nucleotide from, one strand remains stacked and sandwiched between the blunt-ends of two, adjacent Z-DNA duplexes, while the overhanging base of the opposing strand, is extra-helical. The stacked and the extra-helical bases from adjacent, duplexes pair to form a distorted d(G x G) reverse Hoogsteen base-pair in, the d(Gm5CGCGCG)2 homoduplex, and d(G x T) reverse wobble and d(G x C), reverse Watson-Crick base-pairs in the d(GCGCGCG)/d(TCGCGCG) and, d(GCGCGCG)/d(CCGCGCG) heteroduplexes, respectively. Interestingly, only, the d(G,T) and d(G x C) base-pairs were observed in the heteroduplexes, suggesting that both the d(G x T) reverse wobble and d(G x C) reverse, Watson-Crick base-pairs are more stable in this crystal environment than, the d(G x G) reverse Hoogsteen base-pair. To estimate the relative, stability of the three types of reverse base-pairs, crystals were grown, using various mixtures of sequences and their strand compositions analyzed, by mass spectrometry. The d(G x C) reverse Watson-Crick base-pair was, estimated to be more stable by approximately 1.5 kcal/mol and the d(G x T), reverse wobble base-pair more stable by approximately 0.5 kcal/mol than, the d(G x G) reverse Hoogsteen base-pair. The step during crystallization, responsible for discriminating between the strands in the crystal is, highly cooperative, suggesting that it occurs during the initial, nucleating event of crystal growth.

==About this Structure==

313D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=NCO:'>NCO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=313D OCA].

 

The structures and relative stabilities of d(G x G) reverse Hoogsteen, d(G x T) reverse wobble, and d(G x C) reverse Watson-Crick base-pairs in DNA crystals., Mooers BH, Eichman BF, Ho PS, J Mol Biol. 1997 Jun 27;269(5):796-810. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9223642 9223642]

[[Category: Protein complex]]

[[Category: Eichman, B.F.]]

[[Category: Ho, P.S.]]

[[Category: Mooers, B.H.M.]]