328d

[[Image:328d.jpg|left|200px]]<br /><applet load="328d" size="350" color="white" frame="true" align="right" spinBox="true"

caption="328d, resolution 2.600&Aring;" />

'''STRUCTURE OF A D(CGCGAATTCGCG)2-SN7167 COMPLEX'''<br />

==Overview==

The X-ray crystal structure of the complex between the synthetic, antitumour and antiviral DNA binding ligand SN 7167 and the DNA, oligonucleotide d(CGCGAATTCGCG)2 has been determined to an R factor of, 18.3% at 2.6 A resolution. The ligand is located within the minor groove, and covers almost 6 bp with the 1-methylpyridinium ring extending as far, as the C9-G16 base pair and the 1-methylquinolinium ring lying between the, G4-C21 and A5-T20 base pairs. The ligand interacts only weakly with the, DNA, as evidenced by long range contacts and shallow penetration into the, groove. This structure is compared with that of the complex between the, parent compound SN 6999 and the alkylated DNA sequence, d(CGC[e6G]AATTCGCG)2. There are significant differences between the two, structures in the extent of DNA bending, ligand conformation and groove, binding.

 

==About this Structure==

328D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=SN7:'>SN7</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=328D OCA].

 

Minor groove binding of a bis-quaternary ammonium compound: the crystal structure of SN 7167 bound to d(CGCGAATTCGCG)2., Squire CJ, Clark GR, Denny WA, Nucleic Acids Res. 1997 Oct 15;25(20):4072-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9321660 9321660]

[[Category: Protein complex]]

[[Category: Clark, G.R.]]

[[Category: Denny, W.A.]]

[[Category: Squire, C.J.]]

[[Category: SN7]]

[[Category: b-dna]]

[[Category: complexed with drug]]