333d

[[Image:333d.gif|left|200px]]<br /><applet load="333d" size="350" color="white" frame="true" align="right" spinBox="true"

caption="333d, resolution 2.520&Aring;" />

'''THE CRYSTAL STRUCTURE OF AN RNA OLIGOMER INCORPORATING TANDEM ADENOSINE-INOSINE MISMATCHES'''<br />

==Overview==

The X-ray crystallographic structure of the RNA duplex [r(CGCAIGCG)]2 has, been refined to 2.5 A. It shows a symmetric internal loop of two, non-Watson-Crick base pairs which form in the middle of the duplex. The, tandem A-I/I-A pairs are related by a crystallographic two-fold axis. Both, A(anti)-I(anti) mismatches are in a head-to-head conformation forming, hydrogen bonds using the Watson-Crick positions. The octamer duplexes, stack above one another in the cell forming a pseudo-infinite helix, throughout the crystal. A hydrated calcium ion bridges between the, 3'-terminal of one molecule and the backbone of another. The tandem A-I, mismatches are incorporated with only minor distortion to the backbone., This is in contrast to the large helical perturbations often produced by, sheared G-A pairs in RNA oligonucleotides.

 

==About this Structure==

333D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=333D OCA].

 

The crystal structure of an RNA oligomer incorporating tandem adenosine-inosine mismatches., Carter RJ, Baeyens KJ, SantaLucia J, Turner DH, Holbrook SR, Nucleic Acids Res. 1997 Oct 15;25(20):4117-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9321667 9321667]

[[Category: Baeyens, K.J.]]

[[Category: Carter, R.J.]]

[[Category: Holbrook, S.R.]]

[[Category: Jr., J.SantaLucia.]]

[[Category: Turner, D.H.]]

[[Category: CA]]

[[Category: a-rna]]

[[Category: double helix]]

[[Category: internal loop]]

[[Category: mismatched]]