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336d

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INTERACTION BETWEEN LEFT-HANDED Z-DNA AND POLYAMINE-3 THE CRYSTAL STRUCTURE OF THE D(CG)3 AND THERMOSPERMINE COMPLEX

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Retrieved from "http://52.214.119.220/wiki/index.php/336d"

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-[[Image:336d.gif|left|200px]]<br /><applet load="336d" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="336d, resolution 1.000&Aring;" />
-'''INTERACTION BETWEEN LEFT-HANDED Z-DNA AND POLYAMINE-3 THE CRYSTAL STRUCTURE OF THE D(CG)3 AND THERMOSPERMINE COMPLEX'''<br />
-==Overview==
+==INTERACTION BETWEEN LEFT-HANDED Z-DNA AND POLYAMINE-3 THE CRYSTAL STRUCTURE OF THE D(CG)3 AND THERMOSPERMINE COMPLEX==
-The DNA fragment, d(CG)3, was co-crystallized with, N-(3-amino-propyl)-N-(5-aminopropyl)-l,4 -diaminobutane (thermospermine;, PA(334)), a polyamine metabolized from the nucleic acid. By using a good, crystal with dimensions of 0.5 x 0.5 x 0.5 mm3, X-ray intensity data were, collected up to 1.0 A resolution. Two thermospermine molecules and a, magnesium cation were bound to the left-handed double-helical d(CG)3, molecule. The d(CG)3 molecule adopted the left-handed Z-conformation and, two thermospermine molecules and a magnesium cation neutralized the, negative charges of the phosphate groups of the d(CG)3 molecule., Furthermore, the binding modes between d(CG)3 and thermospermine were, different from those of d(CG)3 complexes with PA(24), spermidine and, spermine. This is the first case in which it was determined by X-ray, crystallographic analysis that one of two thermospermine molecules bound, three d(CG)3 duplexes which were symmetrically related to each other, and, the other formed two hydrogen bonds at the N(5) and N(9) atoms with two, adjacent nucleotide phosphate groups of a single d(CG)3 strand at the, minor groove. Furthermore, no direct coordination bond was found between, the d(CG)3 molecule and the magnesium cation.
+<StructureSection load='336d' size='340' side='right'caption='[[336d]], [[Resolution|resolution]] 1.00&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[336d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=336D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=336D FirstGlance]. <br>
-D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=TER:'>TER</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=336D OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TER:N-(3-AMINO-PROPYL)-N-(5-AMINOPROPYL)-1,4-DIAMINOBUTANE'>TER</scene></td></tr>
-==Reference==
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=336d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=336d OCA], [https://pdbe.org/336d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=336d RCSB], [https://www.ebi.ac.uk/pdbsum/336d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=336d ProSAT]</span></td></tr>
-Interaction between left-handed Z-DNA and polyamine - 3. The crystal structure of the d(CG)3 and thermospermine complex., Ohishi H, Terasoma N, Nakanishi I, van der Marel G, van Boom JH, Rich A, Wang AH, Hakoshima T, Tomita K, FEBS Lett. 1996 Dec 2;398(2-3):291-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8977125 8977125]
+</table>
-[[Category: Protein complex]]
+__TOC__
-[[Category: Boom, J.H.Van.]]
+</StructureSection>
-[[Category: Hakoshima, T.]]
+[[Category: Large Structures]]
-[[Category: Marel, G.Van.Der.]]
+[[Category: Hakoshima T]]
-[[Category: Nakanishi, I.]]
+[[Category: Nakanishi I]]
-[[Category: Ohishi, H.]]
+[[Category: Ohishi H]]
-[[Category: Rich, A.]]
+[[Category: Rich A]]
-[[Category: Terasoma, N.]]
+[[Category: Terasoma N]]
-[[Category: Tomita, K.I.]]
+[[Category: Tomita K-I]]
-[[Category: Wang, A.H.J.]]
+[[Category: Van Boom JH]]
-[[Category: MG]]
+[[Category: Van Der Marel G]]
-[[Category: TER]]
+[[Category: Wang AH-J]]
-[[Category: double helix]]
-[[Category: z-dna]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:35:47 2008''

336d

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INTERACTION BETWEEN LEFT-HANDED Z-DNA AND POLYAMINE-3 THE CRYSTAL STRUCTURE OF THE D(CG)3 AND THERMOSPERMINE COMPLEX

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