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349d

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(New page: 200px<br /><applet load="349d" size="350" color="white" frame="true" align="right" spinBox="true" caption="349d, resolution 1.900&Aring;" /> '''X-RAY CRYSTAL STRUC...)
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[[Image:349d.gif|left|200px]]<br /><applet load="349d" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="349d, resolution 1.900&Aring;" />
 
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'''X-RAY CRYSTAL STRUCTURES OF THE DECAMER DGACCGCGGTC: HIGH SALT CONCENTRATION'''<br />
 
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==Overview==
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==X-RAY CRYSTAL STRUCTURES OF THE DECAMER DGACCGCGGTC: HIGH SALT CONCENTRATION==
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The X-ray crystal structure of the DNA decamer d(GACCGCGGTC), containing, half the human papilloma virus E2 binding site, has been solved from two, crystals grown at different ionic conditions (50 mM MgCl2and 50 mM, spermine or 1.56 mM MgCl2and 1.56 mM spermine). Despite the variation in, salt concentration, the two DNA structures are in a very similar, A-type, DNA conformation, with helical axes curving towards the major groove., Although the salt concentrations do not effect the helical parameters or, hydration to a large degree, there is a change in the overall helical, curvature; 18 degrees and 31 degrees for the low and high salt structures, respectively. This curvature appears to be sequence specific and, biologically relevant when compared with similar DNA structures, including, the E2 binding site of a protein-DNA complex.
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<StructureSection load='349d' size='340' side='right'caption='[[349d]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[349d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=349D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=349D FirstGlance]. <br>
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349D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=349D OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=349d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=349d OCA], [https://pdbe.org/349d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=349d RCSB], [https://www.ebi.ac.uk/pdbsum/349d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=349d ProSAT]</span></td></tr>
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==Reference==
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</table>
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X-ray crystal structures of half the human papilloma virus E2 binding site: d(GACCGCGGTC)., Finley JB, Luo M, Nucleic Acids Res. 1998 Dec 15;26(24):5719-27. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9838004 9838004]
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__TOC__
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[[Category: Protein complex]]
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</StructureSection>
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[[Category: Finley, J.B.]]
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[[Category: Large Structures]]
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[[Category: Luo, M.]]
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[[Category: Finley JB]]
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[[Category: a-dna]]
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[[Category: Luo M]]
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[[Category: double helix]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:36:13 2008''
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Current revision

X-RAY CRYSTAL STRUCTURES OF THE DECAMER DGACCGCGGTC: HIGH SALT CONCENTRATION

PDB ID 349d

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