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359d

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(New page: 200px<br /><applet load="359d" size="350" color="white" frame="true" align="right" spinBox="true" caption="359d, resolution 2.900&Aring;" /> '''INHIBITION OF THE H...)
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[[Image:359d.jpg|left|200px]]<br /><applet load="359d" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="359d, resolution 2.900&Aring;" />
 
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'''INHIBITION OF THE HAMMERHEAD RIBOZYME CLEAVAGE REACTION BY SITE-SPECIFIC BINDING OF TB(III)'''<br />
 
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==Overview==
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==INHIBITION OF THE HAMMERHEAD RIBOZYME CLEAVAGE REACTION BY SITE-SPECIFIC BINDING OF TB(III)==
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Terbium(III) [Tb(III)] was shown to inhibit the hammerhead ribozyme by, competing with a single magnesium(II) ion. X-ray crystallography revealed, that the Tb(III) ion binds to a site adjacent to an essential guanosine in, the catalytic core of the ribozyme, approximately 10 angstroms from the, cleavage site. Synthetic modifications near this binding site yielded an, RNA substrate that was resistant to Tb(III) binding and capable of being, cleaved, even in the presence of up to 20 micromolar Tb(III). It is, suggested that the magnesium(II) ion thought to bind at this site may act, as a switch, affecting the conformational changes required to achieve the, transition state.
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<StructureSection load='359d' size='340' side='right'caption='[[359d]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[359d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=359D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=359D FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TB:TERBIUM(III)+ION'>TB</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=359d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=359d OCA], [https://pdbe.org/359d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=359d RCSB], [https://www.ebi.ac.uk/pdbsum/359d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=359d ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Terbium(III) [Tb(III)] was shown to inhibit the hammerhead ribozyme by competing with a single magnesium(II) ion. X-ray crystallography revealed that the Tb(III) ion binds to a site adjacent to an essential guanosine in the catalytic core of the ribozyme, approximately 10 angstroms from the cleavage site. Synthetic modifications near this binding site yielded an RNA substrate that was resistant to Tb(III) binding and capable of being cleaved, even in the presence of up to 20 micromolar Tb(III). It is suggested that the magnesium(II) ion thought to bind at this site may act as a switch, affecting the conformational changes required to achieve the transition state.
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==About this Structure==
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Inhibition of the hammerhead ribozyme cleavage reaction by site-specific binding of Tb.,Feig AL, Scott WG, Uhlenbeck OC Science. 1998 Jan 2;279(5347):81-4. PMID:9417029<ref>PMID:9417029</ref>
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359D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=TB:'>TB</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=359D OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Inhibition of the hammerhead ribozyme cleavage reaction by site-specific binding of Tb., Feig AL, Scott WG, Uhlenbeck OC, Science. 1998 Jan 2;279(5347):81-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9417029 9417029]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 359d" style="background-color:#fffaf0;"></div>
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[[Category: Feig, A.L.]]
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[[Category: Scott, W.G.]]
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[[Category: Uhlenbeck, O.C.]]
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[[Category: TB]]
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[[Category: catalytic rna]]
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[[Category: loop]]
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[[Category: rna hammerhead ribozyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:36:35 2008''
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==See Also==
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*[[Ribozyme 3D structures|Ribozyme 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Feig AL]]
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[[Category: Scott WG]]
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[[Category: Uhlenbeck OC]]

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INHIBITION OF THE HAMMERHEAD RIBOZYME CLEAVAGE REACTION BY SITE-SPECIFIC BINDING OF TB(III)

PDB ID 359d

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