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1h8y

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[[Image:1h8y.gif|left|200px]]<br />
 
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<applet load="1h8y" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1h8y, resolution 2.0&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE CLASS D BETA-LACTAMASE OXA-13 IN COMPLEX WITH MEROPENEM'''<br />
 
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==Overview==
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==Crystal structure of the class D beta-lactamase OXA-13 in complex with meropenem==
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The therapeutic problems posed by class D beta-lactamases, a family of, serine enzymes that hydrolyse beta-lactam antibiotics following an, acylation-deacylation mechanism, are increased by the very low level of, sensitivity of these enzymes to beta-lactamase inhibitors. To gain, structural and mechanistic insights to aid the design of new inhibitors, we have determined the crystal structure of OXA-13 from Pseudomonas, aeruginosa in the apo form and in complex with the carbapenem meropenem., The native form consisted of a dimer displaying an overall organisation, similar to that found in the closely related enzyme OXA-10. In the, acyl-enzyme complex, the positioning of the antibiotic appeared to be, ensured mainly by (i) the covalent acyl bond and (ii) a strong salt-bridge, involving the ... [[http://ispc.weizmann.ac.il/pmbin/getpm?11453693 (full description)]]
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<StructureSection load='1h8y' size='340' side='right'caption='[[1h8y]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1h8y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H8Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H8Y FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MER:(4R,5S)-3-{[(3S,5S)-5-(DIMETHYLCARBAMOYL)PYRROLIDIN-3-YL]SULFANYL}-5-[(2S,3R)-3-HYDROXY-1-OXOBUTAN-2-YL]-4-METHYL-4,5-DIHYDRO-1H-PYRROLE-2-CARBOXYLIC+ACID'>MER</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h8y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h8y OCA], [https://pdbe.org/1h8y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h8y RCSB], [https://www.ebi.ac.uk/pdbsum/1h8y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h8y ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q51400_PSEAI Q51400_PSEAI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h8/1h8y_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h8y ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1H8Y is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]] with SO4 and MER as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6]]. Structure known Active Sites: AC1 and AC2. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H8Y OCA]].
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*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structures of the class D beta-lactamase OXA-13 in the native form and in complex with meropenem., Pernot L, Frenois F, Rybkine T, L'Hermite G, Petrella S, Delettre J, Jarlier V, Collatz E, Sougakoff W, J Mol Biol. 2001 Jul 20;310(4):859-74. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11453693 11453693]
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[[Category: Large Structures]]
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[[Category: Beta-lactamase]]
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[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Single protein]]
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[[Category: Collatz E]]
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[[Category: Collatz, E.]]
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[[Category: Delettre J]]
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[[Category: Delettre, J.]]
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[[Category: Frenois F]]
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[[Category: Frenois, F.]]
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[[Category: Jarlier V]]
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[[Category: Hermite, G.L.]]
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[[Category: L'Hermite G]]
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[[Category: Jarlier, V.]]
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[[Category: Pernot L]]
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[[Category: Pernot, L.]]
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[[Category: Petrella S]]
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[[Category: Petrella, S.]]
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[[Category: Rybkine T]]
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[[Category: Rybkine, T.]]
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[[Category: Sougakoff W]]
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[[Category: Sougakoff, W.]]
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[[Category: MER]]
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[[Category: SO4]]
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[[Category: acyl-enzyme]]
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[[Category: beta-lactamase]]
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[[Category: class d]]
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[[Category: hydrolase]]
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[[Category: meropenem]]
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[[Category: oxa-13]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:22:56 2007''
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Current revision

Crystal structure of the class D beta-lactamase OXA-13 in complex with meropenem

PDB ID 1h8y

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