408d

[[Image:408d.gif|left|200px]]<br /><applet load="408d" size="350" color="white" frame="true" align="right" spinBox="true"

caption="408d, resolution 2.100&Aring;" />

'''STRUCTURAL BASIS FOR RECOGNITION OF A-T AND T-A BASE PAIRS IN THE MINOR GROOVE OF B-DNA'''<br />

==Overview==

Polyamide dimers containing three types of aromatic rings-pyrrole, imidazole, and hydroxypyrrole-afford a small-molecule recognition code, that discriminates among all four Watson-Crick base pairs in the minor, groove. The crystal structure of a specific polyamide dimer-DNA complex, establishes the structural basis for distinguishing T.A from A.T base, pairs. Specificity for the T.A base pair is achieved by means of distinct, hydrogen bonds between pairs of substituted pyrroles on the ligand and the, O2 of thymine and N3 of adenine. In addition, shape-selective recognition, of an asymmetric cleft between the thymine-O2 and the adenine-C2 was, observed. Although hitherto similarities among the base pairs in the minor, groove have been emphasized, the structure illustrates differences that, allow specific minor groove recognition.

 

==About this Structure==

408D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=IPY:'>IPY</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=408D OCA].

 

A structural basis for recognition of A.T and T.A base pairs in the minor groove of B-DNA., Kielkopf CL, White S, Szewczyk JW, Turner JM, Baird EE, Dervan PB, Rees DC, Science. 1998 Oct 2;282(5386):111-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9756473 9756473]

[[Category: Protein complex]]

[[Category: Baird, E.E.]]

[[Category: Dervan, P.B.]]

[[Category: Kielkopf, C.L.]]

[[Category: Rees, D.C.]]

[[Category: Szewczyk, J.W.]]

[[Category: Turner, J.M.]]

[[Category: White, S.]]