This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2iu5

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:29, 9 May 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2iu5.png|left|200px]]
 
-
<!--
+
==Dihydroxyacetone kinase operon activator DhaS==
-
The line below this paragraph, containing "STRUCTURE_2iu5", creates the "Structure Box" on the page.
+
<StructureSection load='2iu5' size='340' side='right'caption='[[2iu5]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2iu5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis_Il1403 Lactococcus lactis subsp. lactis Il1403]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IU5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IU5 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2iu5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iu5 OCA], [https://pdbe.org/2iu5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2iu5 RCSB], [https://www.ebi.ac.uk/pdbsum/2iu5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2iu5 ProSAT]</span></td></tr>
-
{{STRUCTURE_2iu5| PDB=2iu5 | SCENE= }}
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/DHAS_LACLA DHAS_LACLA] In complex with DhaQ, upon activation by dihydroxyacetone, activates transcription of the dhaKLM operon. Binds the inverted repeat sequence 5'-GGACACATN(6)ATTTGTCC-3' located upstream of and partially overlapping with the -35 promoter sequence of the dhaKLM operon promoter.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iu/2iu5_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2iu5 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
Dihydroxyacetone (Dha) kinases are a novel family of kinases with signaling and metabolic functions. Here we report the x-ray structures of the transcriptional activator DhaS and the coactivator DhaQ and characterize their function. DhaQ is a paralog of the Dha binding Dha kinase subunit; DhaS belongs to the family of TetR repressors although, unlike all known members of this family, it is a transcriptional activator. DhaQ and DhaS form a stable complex that in the presence of Dha activates transcription of the Lactococcus lactis dha operon. Dha covalently binds to DhaQ through a hemiaminal bond with a histidine and thereby induces a conformational change, which is propagated to the surface via a cantilever-like structure. DhaS binding protects an inverted repeat whose sequence is GGACACATN6ATTTGTCC and renders two GC base pairs of the operator DNA hypersensitive to DNase I cleavage. The proximal half-site of the inverted repeat partially overlaps with the predicted -35 consensus sequence of the dha promoter.
-
===DIHYDROXYACETONE KINASE OPERON ACTIVATOR DHAS===
+
Regulation of the Dha operon of Lactococcus lactis: a deviation from the rule followed by the Tetr family of transcription regulators.,Christen S, Srinivas A, Bahler P, Zeller A, Pridmore D, Bieniossek C, Baumann U, Erni B J Biol Chem. 2006 Aug 11;281(32):23129-37. Epub 2006 Jun 7. PMID:16760471<ref>PMID:16760471</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_16760471}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 2iu5" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 16760471 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_16760471}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Lactococcus lactis subsp. lactis Il1403]]
-
[[2iu5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis_il1403 Lactococcus lactis subsp. lactis il1403]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IU5 OCA].
+
[[Category: Large Structures]]
-
 
+
[[Category: Baumann U]]
-
==Reference==
+
[[Category: Christen S]]
-
<ref group="xtra">PMID:016760471</ref><references group="xtra"/>
+
[[Category: Erni B]]
-
[[Category: Lactococcus lactis subsp. lactis il1403]]
+
[[Category: Srinivas A]]
-
[[Category: Baumann, U.]]
+
-
[[Category: Christen, S.]]
+
-
[[Category: Erni, B.]]
+
-
[[Category: Srinivas, A.]]
+
-
[[Category: Activator]]
+
-
[[Category: Synthase]]
+
-
[[Category: Tetr family]]
+
-
[[Category: Transcription]]
+

Current revision

Dihydroxyacetone kinase operon activator DhaS

PDB ID 2iu5

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2iu5"
Personal tools