430d

[[Image:430d.gif|left|200px]]<br /><applet load="430d" size="350" color="white" frame="true" align="right" spinBox="true"

caption="430d, resolution 2.10&Aring;" />

'''STRUCTURE OF SARCIN/RICIN LOOP FROM RAT 28S RRNA'''<br />

==Overview==

The structure of a 29-nucleotide RNA containing the sarcin/ricin loop, (SRL) of rat 28 S rRNA has been determined at 2.1 A resolution., Recognition of the SRL by elongation factors and by the ribotoxins, sarcin, and ricin, requires a nearly universal dodecamer sequence that folds into, a G-bulged cross-strand A stack and a GAGA tetraloop. The juxtaposition of, these two motifs forms a distorted hairpin structure that allows direct, recognition of bases in both grooves as well as recognition of nonhelical, backbone geometry and two 5'-unstacked purines. Comparisons with other RNA, crystal structures establish the cross-strand A stack and the GNRA, tetraloop as defined and modular RNA structural elements. The conserved, region at the top is connected to the base of the domain by a region, presumed to be flexible because of the sparsity of stabilizing contacts., Although the conformation of the SRL RNA previously determined by NMR, spectroscopy is similar to the structure determined by x-ray, crystallography, significant differences are observed in the "flexible", region and to a lesser extent in the G-bulged cross-strand A stack.

 

==About this Structure==

430D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=430D OCA].

 

Crystal structure of the ribosomal RNA domain essential for binding elongation factors., Correll CC, Munishkin A, Chan YL, Ren Z, Wool IG, Steitz TA, Proc Natl Acad Sci U S A. 1998 Nov 10;95(23):13436-41. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9811818 9811818]

[[Category: Chan, Y-L.]]

[[Category: Correll, C.C.]]

[[Category: Munishkin, A.]]

[[Category: Ren, Z.]]

[[Category: Steitz, T.A.]]

[[Category: MG]]

[[Category: base triple]]

[[Category: blunt stem]]

[[Category: double helix]]

[[Category: hairpin]]

[[Category: ribonucleic acid]]

[[Category: tetraloop mismatched]]

[[Category: u-rna]]