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441d

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HIGH RESOLUTION A-DNA CRYSTAL STRYCTURES OF D(AGGGGCCCCT): AN A-DNA MODEL OF POLY(DG).POLY(DC)

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Retrieved from "http://52.214.119.220/wiki/index.php/441d"

Categories: Large Structures | Synthetic construct | Gao YG | Robinson HH | Wang AH

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-[[Image:441d.gif|left|200px]]<br /><applet load="441d" size="350" color="white" frame="true" align="right" spinBox="true"
-caption="441d, resolution 1.50&Aring;" />
-'''HIGH RESOLUTION A-DNA CRYSTAL STRYCTURES OF D(AGGGGCCCCT): AN A-DNA MODEL OF POLY(DG).POLY(DC)'''<br />
-==Overview==
+==HIGH RESOLUTION A-DNA CRYSTAL STRYCTURES OF D(AGGGGCCCCT): AN A-DNA MODEL OF POLY(DG).POLY(DC)==
-A-DNA conformation is favored by guanine-rich sequences, such as (dG)n x, (dC)n, or under low-humidity conditions. Earlier A-DNA crystal structures, revealed some conformational variations which may be the result of, sequence-dependent effects and/or crystal packing forces. Here we report, the high-resolution crystal structure of d(AGGGGCCCCT) in two crystal, forms (either in the P212121 or the P6122 space group) to gain insights, into the conformation and dynamics of the (dG)n x (dC)n sequence. The, P212121 form has been analyzed using data to 1.1 A resolution by the, anisotropic temperature factor refinement procedure of the SHELX97, program. Such analysis affords us with the detailed geometric, conformational and motional property of an A-DNA structure. The backbone, torsional angles fall in a narrow range, except for the alpha/gamma angles, which have two distinct combinations (gauche-/gauche+ or trans/trans). An, A-DNA model of poly(dG) x poly(dC) has been constructed using the, conformational parameters derived from the crystal structure of the, P212121 form. In the crystal structure of the P6122 space group, the, central eight base pairs of the decamer adopt A-DNA conformation with the, two terminal nucleotides flipped out to form base pairs with the, neighboring nucleotides. Comparison of the A-DNA structure of the same, sequence from two different crystal forms, reinforced the conclusion that, molecules crystallized in the same space group have a more similar, conformation, whereas the same molecule crystallized in different space, groups has different (local) conformations.
+<StructureSection load='441d' size='340' side='right'caption='[[441d]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
+== Structural highlights ==
-==About this Structure==
+<table><tr><td colspan='2'>[[441d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=441D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=441D FirstGlance]. <br>
-D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=441D OCA].
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=441d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=441d OCA], [https://pdbe.org/441d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=441d RCSB], [https://www.ebi.ac.uk/pdbsum/441d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=441d ProSAT]</span></td></tr>
-==Reference==
+</table>
-High-resolution A-DNA crystal structures of d(AGGGGCCCCT). An A-DNA model of poly(dG) x poly(dC)., Gao YG, Robinson H, Wang AH, Eur J Biochem. 1999 Apr;261(2):413-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10215851 10215851]
+__TOC__
-[[Category: Protein complex]]
+</StructureSection>
-[[Category: Gao, Y.G.]]
+[[Category: Large Structures]]
-[[Category: Robinson, H.H.]]
+[[Category: Synthetic construct]]
-[[Category: Wang, A.H-J.]]
+[[Category: Gao YG]]
-[[Category: a-dna]]
+[[Category: Robinson HH]]
-[[Category: double helix]]
+[[Category: Wang AH]]
-[[Category: high resolution]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:43:38 2008''

441d

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HIGH RESOLUTION A-DNA CRYSTAL STRYCTURES OF D(AGGGGCCCCT): AN A-DNA MODEL OF POLY(DG).POLY(DC)

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