2w7z

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[[Image:2w7z.png|left|200px]]
 
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==Structure of the pentapeptide repeat protein EfsQnr, a DNA gyrase inhibitor. Free amines modified by cyclic pentylation with glutaraldehyde.==
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The line below this paragraph, containing "STRUCTURE_2w7z", creates the "Structure Box" on the page.
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<StructureSection load='2w7z' size='340' side='right'caption='[[2w7z]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2w7z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W7Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W7Z FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=LP6:6-PIPERIDIN-1-YL-L-NORLEUCINE'>LP6</scene></td></tr>
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{{STRUCTURE_2w7z| PDB=2w7z | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w7z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w7z OCA], [https://pdbe.org/2w7z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w7z RCSB], [https://www.ebi.ac.uk/pdbsum/2w7z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w7z ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q837D8_ENTFA Q837D8_ENTFA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w7/2w7z_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w7z ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The pentapeptide-repeat protein EfsQnr from Enterococcus faecalis protects DNA gyrase from inhibition by fluoroquinolones. EfsQnr was cloned and purified to homogeneity, but failed to produce diffraction-quality crystals in initial crystallization screens. Treatment of EfsQnr with glutaraldehyde and the strong reducing agent borane-dimethylamine resulted in a derivatized protein which produced crystals that diffracted to 1.6 A resolution; their structure was subsequently determined by single-wavelength anomalous dispersion. Analysis of the derivatized protein using Fourier transform ion cyclotron resonance mass spectrometry indicated a mass increase of 68 Da per free amino group. Electron-density maps about a limited number of structurally ordered lysines indicated that the modification was a cyclic pentylation of free amines, producing piperidine groups.
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===STRUCTURE OF THE PENTAPEPTIDE REPEAT PROTEIN EFSQNR, A DNA GYRASE INHIBITOR. FREE AMINES MODIFIED BY CYCLIC PENTYLATION WITH GLUTARALDEHYDE.===
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Crystallization of a pentapeptide-repeat protein by reductive cyclic pentylation of free amines with glutaraldehyde.,Vetting MW, Hegde SS, Blanchard JS Acta Crystallogr D Biol Crystallogr. 2009 May;65(Pt 5):462-9. Epub 2009, Apr 18. PMID:19390151<ref>PMID:19390151</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_19390151}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2w7z" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 19390151 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_19390151}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[2w7z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W7Z OCA].
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==Reference==
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<ref group="xtra">PMID:019390151</ref><references group="xtra"/>
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[[Category: Enterococcus faecalis]]
[[Category: Enterococcus faecalis]]
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[[Category: Blanchard, J S.]]
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[[Category: Large Structures]]
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[[Category: Hegde, S S.]]
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[[Category: Blanchard JS]]
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[[Category: Vetting, M W.]]
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[[Category: Hegde SS]]
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[[Category: Chemical modification]]
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[[Category: Vetting MW]]
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[[Category: Cyclic pentylation]]
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[[Category: Glutaraldehyde]]
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[[Category: Gyrase inhibitor]]
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[[Category: Inhibitor]]
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[[Category: Pentapeptide repeat protein]]
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Current revision

Structure of the pentapeptide repeat protein EfsQnr, a DNA gyrase inhibitor. Free amines modified by cyclic pentylation with glutaraldehyde.

PDB ID 2w7z

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