2x0k

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[[Image:2x0k.png|left|200px]]
 
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==Crystal structure of modular FAD synthetase from Corynebacterium ammoniagenes==
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The line below this paragraph, containing "STRUCTURE_2x0k", creates the "Structure Box" on the page.
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<StructureSection load='2x0k' size='340' side='right'caption='[[2x0k]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2x0k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_ammoniagenes Corynebacterium ammoniagenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X0K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X0K FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PPV:PYROPHOSPHATE'>PPV</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_2x0k| PDB=2x0k | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x0k OCA], [https://pdbe.org/2x0k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x0k RCSB], [https://www.ebi.ac.uk/pdbsum/2x0k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x0k ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RIBF_CORAM RIBF_CORAM]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x0/2x0k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x0k ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of the modular flavin adenine dinucleotide (FAD) synthetase from Corynebacterium ammoniagenes has been solved at 1.95 A resolution. The structure of C. ammoniagenes FAD synthetase presents two catalytic modules-a C-terminus with ATP-riboflavin kinase activity and an N-terminus with ATP-flavin mononucleotide (FMN) adenylyltransferase activity-that are responsible for the synthesis of FAD from riboflavin in two sequential steps. In the monomeric structure, the active sites from both modules are placed 40 A away, preventing the direct transfer of the product from the first reaction (FMN) to the second catalytic site, where it acts as substrate. Crystallographic and biophysical studies revealed a hexameric assembly formed by the interaction of two trimers. Each trimer presents a head-tail configuration, with FMN adenylyltransferase and riboflavin kinase modules from different protomers approaching the active sites and allowing the direct transfer of FMN. Experimental results provide molecular-level evidences of the mechanism of the synthesis of FMN and FAD in prokaryotes in which the oligomeric state could be involved in the regulation of the catalytic efficiency of the modular enzyme.
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===CRYSTAL STRUCTURE OF MODULAR FAD SYNTHETASE FROM CORYNEBACTERIUM AMMONIAGENES===
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Oligomeric state in the crystal structure of modular FAD synthetase provides insights into its sequential catalysis in prokaryotes.,Herguedas B, Martinez-Julvez M, Frago S, Medina M, Hermoso JA J Mol Biol. 2010 Jul 9;400(2):218-30. Epub 2010 May 13. PMID:20471397<ref>PMID:20471397</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20471397}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2x0k" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20471397 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20471397}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[2x0k]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Corynebacterium_ammoniagenes Corynebacterium ammoniagenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X0K OCA].
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==Reference==
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<ref group="xtra">PMID:020471397</ref><references group="xtra"/>
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[[Category: Corynebacterium ammoniagenes]]
[[Category: Corynebacterium ammoniagenes]]
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[[Category: Frago, S.]]
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[[Category: Large Structures]]
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[[Category: Herguedas, B.]]
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[[Category: Frago S]]
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[[Category: Hermoso, J A.]]
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[[Category: Herguedas B]]
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[[Category: Martinez-Julvez, M.]]
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[[Category: Hermoso JA]]
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[[Category: Medina, M.]]
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[[Category: Martinez-Julvez M]]
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[[Category: Atp-binding]]
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[[Category: Medina M]]
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[[Category: Multifunctional enzyme]]
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[[Category: Nucleotide-binding]]
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[[Category: Nucleotidyltransferase]]
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[[Category: Riboflavin kinase]]
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[[Category: Transferase]]
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Current revision

Crystal structure of modular FAD synthetase from Corynebacterium ammoniagenes

PDB ID 2x0k

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