2c7f

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[[Image:2c7f.png|left|200px]]
 
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==The Structure of a family 51 arabinofuranosidase, Araf51, from Clostridium thermocellum in complex with 1,5-alpha-L-Arabinotriose.==
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The line below this paragraph, containing "STRUCTURE_2c7f", creates the "Structure Box" on the page.
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<StructureSection load='2c7f' size='340' side='right'caption='[[2c7f]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2c7f]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Acetivibrio_thermocellus Acetivibrio thermocellus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C7F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2C7F FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AHR:ALPHA-L-ARABINOFURANOSE'>AHR</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
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{{STRUCTURE_2c7f| PDB=2c7f | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2c7f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2c7f OCA], [https://pdbe.org/2c7f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2c7f RCSB], [https://www.ebi.ac.uk/pdbsum/2c7f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2c7f ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IABF_ACET2 IABF_ACET2] Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the cleavage of terminal alpha-(1->5)-arabinofuranosyl bonds in small oligosaccharides as alpha-(1->5)-linked arabinobiose/arabinotriose, but does not display significant activity against linear non-substituted arabinan. It is also highly efficient in the cleavage of alpha-(1->3)-linked arabinoside of xylobiose and of the alpha-(1->3)-linked arabinoside decorations of polymeric wheat arabinoxylan. It exhibits very low activity against sugar beet arabinan.<ref>PMID:16336192</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c7/2c7f_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2c7f ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The digestion of the plant cell wall requires the concerted action of a diverse repertoire of enzyme activities. An important component of these hydrolase consortia are arabinofuranosidases, which release L-arabinofuranose moieties from a range of plant structural polysaccharides. The anaerobic bacterium Clostridium thermocellum, a highly efficient plant cell wall degrader, possesses a single alpha-L-arabinofuranosidase (EC 3.2.1.55), CtAraf51A, located in GH51 (glycoside hydrolase family 51). The crystal structure of the enzyme has been solved in native form and in 'Michaelis' complexes with both alpha-1,5-linked arabinotriose and alpha-1,3 arabinoxylobiose, both forming a hexamer in the asymmetric unit. Kinetic studies reveal that CtAraf51A, in contrast with well-characterized GH51 enzymes including the Cellvibrio japonicus enzyme [Beylot, McKie, Voragen, Doeswijk-Voragen and Gilbert (2001) Biochem. J. 358, 607-614], catalyses the hydrolysis of alpha-1,5-linked arabino-oligosaccharides and the alpha-1,3 arabinosyl side chain decorations of xylan with equal efficiency. The paucity of direct hydrogen bonds with the aglycone moiety and the flexible conformation adopted by Trp(178), which stacks against the sugar at the +1 subsite, provide a structural explanation for the plasticity in substrate specificity displayed by the clostridial arabinofuranosidase.
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===THE STRUCTURE OF A FAMILY 51 ARABINOFURANOSIDASE, ARAF51, FROM CLOSTRIDIUM THERMOCELLUM IN COMPLEX WITH 1,5-ALPHA-L-ARABINOTRIOSE.===
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Structural insight into the ligand specificity of a thermostable family 51 arabinofuranosidase, Araf51, from Clostridium thermocellum.,Taylor EJ, Smith NL, Turkenburg JP, D'Souza S, Gilbert HJ, Davies GJ Biochem J. 2006 Apr 1;395(1):31-7. PMID:16336192<ref>PMID:16336192</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_16336192}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2c7f" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 16336192 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_16336192}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Acetivibrio thermocellus]]
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[[2c7f]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_thermocellum Clostridium thermocellum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C7F OCA].
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[[Category: Large Structures]]
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[[Category: D'Souza S]]
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==Reference==
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[[Category: Davies GJ]]
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<ref group="xtra">PMID:016336192</ref><references group="xtra"/>
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[[Category: Gilbert HJ]]
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[[Category: Alpha-N-arabinofuranosidase]]
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[[Category: Smith NL]]
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[[Category: Clostridium thermocellum]]
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[[Category: Taylor EJ]]
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[[Category: Davies, G J.]]
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[[Category: Turkenburg JP]]
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[[Category: Gilbert, H J.]]
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[[Category: Smith, N L.]]
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[[Category: Souza, S D.]]
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[[Category: Taylor, E J.]]
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[[Category: Turkenburg, J P.]]
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[[Category: Arabinan]]
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[[Category: Arabinofuranosidase]]
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[[Category: Glycosidase]]
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[[Category: Hydrolase]]
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[[Category: Xylan]]
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Current revision

The Structure of a family 51 arabinofuranosidase, Araf51, from Clostridium thermocellum in complex with 1,5-alpha-L-Arabinotriose.

PDB ID 2c7f

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