482d

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(New page: 200px<br /><applet load="482d" size="350" color="white" frame="true" align="right" spinBox="true" caption="482d, resolution 1.54&Aring;" /> '''RELEASE OF THE CYANO...)
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[[Image:482d.gif|left|200px]]<br /><applet load="482d" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="482d, resolution 1.54&Aring;" />
 
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'''RELEASE OF THE CYANO MOIETY IN THE CRYSTAL STRUCTURE OF N-CYANOMETHYL-N-(2-METHOXYETHYL)-DAUNOMYCIN COMPLEXED WITH D(CGATCG)'''<br />
 
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==Overview==
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==RELEASE OF THE CYANO MOIETY IN THE CRYSTAL STRUCTURE OF N-CYANOMETHYL-N-(2-METHOXYETHYL)-DAUNOMYCIN COMPLEXED WITH D(CGATCG)==
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Doxorubicin is among the most widely used anthracycline in cancer, chemotherapy. In an attempt to avoid the cardiotoxicity and drug, resistance of doxorubicin therapy, several analogues were synthesized. The, cyanomorpholinyl derivative is the most cytotoxic. They differ greatly, from their parent compound in their biological and pharmacological, properties, inducing cross-links in drug DNA complexes. The present study, concerns N-cyanomethyl-N-(2-methoxyethyl)-daunomycin (CMDa), a synthetic, analogue of cyanomorpholino-daunomycin. Compared to doxorubicin, CMDa, displays a cytotoxic activity on L1210 leukemia cells at higher, concentration but is effective on doxorubicin resistant cells. The results, of fluorescence quenching experiments as well as the melting temperature, (DeltaTm = 7.5 degrees C) studies are consistent with a drug molecule, which intercalates between the DNA base pairs and stabilizes the DNA, double helix. The crystal structure of CMDa complexed to the, hexanucleotide d(CGATCG) has been determined at 1.5 A resolution. The, complex crystallizes in the space group P41212 and is similar to other, anthracycline-hexanucleotide complexes. In the crystal state, the observed, densities indicate the formation of, N-hydroxymethyl-N-(2-methoxyethyl)-daunomycin (HMDa) with the release of, the cyano moiety without DNA alkylation. The formation of this degradation, compound is discussed in relation with other drug modifications when, binding to DNA. Comparison with two other drug-DNA crystal structures, suggests a correlation between a slight change in DNA conformation and the, nature of the amino sugar substituents at the N3' position located in the, minor groove.
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<StructureSection load='482d' size='340' side='right'caption='[[482d]], [[Resolution|resolution]] 1.54&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[482d]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=482D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=482D FirstGlance]. <br>
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482D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=DM9:'>DM9</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=482D OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.54&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DM9:N-HYDROXYMETHYL-N-(2-METHOXYETHYL)-DAUNOMYCIN'>DM9</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=482d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=482d OCA], [https://pdbe.org/482d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=482d RCSB], [https://www.ebi.ac.uk/pdbsum/482d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=482d ProSAT]</span></td></tr>
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Release of the cyano moiety in the crystal structure of N-cyanomethyl-N-(2-methoxyethyl)-daunomycin complexed with d(CGATCG)., Saminadin P, Dautant A, Mondon M, Langlois D'estaintot B, Courseille C, Precigoux G, Eur J Biochem. 2000 Jan;267(2):457-64. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10632715 10632715]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Courseille, C.]]
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</StructureSection>
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[[Category: Dautant, A.]]
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[[Category: Large Structures]]
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[[Category: Estaintot, B.Langlois.D.]]
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[[Category: Courseille C]]
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[[Category: Mondon, M.]]
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[[Category: Dautant A]]
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[[Category: Precigoux, G.]]
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[[Category: Langlois D'Estaintot B]]
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[[Category: Saminadin, P.]]
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[[Category: Mondon M]]
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[[Category: DM9]]
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[[Category: Precigoux G]]
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[[Category: complexed with drug]]
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[[Category: Saminadin P]]
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[[Category: deoxyribonucleic acid]]
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[[Category: double helix]]
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[[Category: right handed dna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:45:15 2008''
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Current revision

RELEASE OF THE CYANO MOIETY IN THE CRYSTAL STRUCTURE OF N-CYANOMETHYL-N-(2-METHOXYETHYL)-DAUNOMYCIN COMPLEXED WITH D(CGATCG)

PDB ID 482d

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