4hb1

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="4hb1" size="350" color="white" frame="true" align="right" spinBox="true" caption="4hb1, resolution 2.9&Aring;" /> '''A DESIGNED FOUR HELIX...)
Current revision (11:36, 1 March 2024) (edit) (undo)
 
(11 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:4hb1.jpg|left|200px]]<br /><applet load="4hb1" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="4hb1, resolution 2.9&Aring;" />
 
-
'''A DESIGNED FOUR HELIX BUNDLE PROTEIN.'''<br />
 
-
==Overview==
+
==A DESIGNED FOUR HELIX BUNDLE PROTEIN.==
-
A 108 amino acid protein was designed and constructed from a reduced, alphabet of seven amino acids. The 2.9 A resolution X-ray crystal, structure confirms that the protein is a four helix bundle, as it was, designed to be. Hydrogen/deuterium exchange experiments reveal buried, amide protons with protection factors in excess of 1 x 10(6) in the range, characteristic of well protected protons in functional folded proteins, (10(3)-10(8)) rather than protons in rapid exchange (0-10(2)). The protein, is monomeric at 1 mM, the concentration at which the exchange experiments, were undertaken, indicating that the exchange factors are due to a unique, stable tertiary structure fold, and not due to any higher order quaternary, structure. Thermodynamic analysis provides an estimate of the free energy, of folding of -9.3 kcal mole-1 at 25 degrees C, consistent with the free, energy of folding derived from the protection factors of the most, protected protons, indicating that global unfolding is required for, exchange of the most protected protons.
+
<StructureSection load='4hb1' size='340' side='right'caption='[[4hb1]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[4hb1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HB1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HB1 FirstGlance]. <br>
-
4HB1 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct] with <scene name='pdbligand=UNX:'>UNX</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HB1 OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hb1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hb1 OCA], [https://pdbe.org/4hb1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hb1 RCSB], [https://www.ebi.ac.uk/pdbsum/4hb1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hb1 ProSAT]</span></td></tr>
-
A designed four helix bundle protein with native-like structure., Schafmeister CE, LaPorte SL, Miercke LJ, Stroud RM, Nat Struct Biol. 1997 Dec;4(12):1039-46. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9406555 9406555]
+
</table>
-
[[Category: Protein complex]]
+
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Synthetic construct]]
-
[[Category: Laporte, S.L.]]
+
[[Category: Laporte SL]]
-
[[Category: Miercke, L.J.W.]]
+
[[Category: Miercke LJW]]
-
[[Category: Schafmeister, C.E.]]
+
[[Category: Schafmeister CE]]
-
[[Category: Stroud, R.M.]]
+
[[Category: Stroud RM]]
-
[[Category: UNX]]
+
-
[[Category: designed helical bundle]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:46:33 2008''
+

Current revision

A DESIGNED FOUR HELIX BUNDLE PROTEIN.

PDB ID 4hb1

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4hb1"
Personal tools