2cnm

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[[Image:2cnm.png|left|200px]]
 
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==RimI - Ribosomal S18 N-alpha-protein acetyltransferase in complex with a bisubstrate inhibitor (Cterm-Arg-Arg-Phe-Tyr-Arg-Ala-N-alpha- acetyl-S-CoA).==
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The line below this paragraph, containing "STRUCTURE_2cnm", creates the "Structure Box" on the page.
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<StructureSection load='2cnm' size='340' side='right'caption='[[2cnm]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2cnm]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CNM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CNM FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr>
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{{STRUCTURE_2cnm| PDB=2cnm | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cnm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cnm OCA], [https://pdbe.org/2cnm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cnm RCSB], [https://www.ebi.ac.uk/pdbsum/2cnm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cnm ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RIMI_SALTY RIMI_SALTY] Acetylates the N-terminal alanine of ribosomal protein bS18.[HAMAP-Rule:MF_02210]<ref>PMID:18596200</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cn/2cnm_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cnm ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The three ribosomal proteins L7, S5, and S18 are included in the rare subset of prokaryotic proteins that are known to be N(alpha)-acetylated. The GCN5-related N-acetyltransferase (GNAT) protein RimI, responsible for the N(alpha)-acetylation of the ribosomal protein S18, was cloned from Salmonella typhimurium LT2 (RimI(ST)), overexpressed, and purified to homogeneity. Steady-state kinetic parameters for RimI(ST) were determined for AcCoA and a peptide substrate consisting of the first six amino acids of the target protein S18. The crystal structure of RimI(ST) was determined in complex with CoA, AcCoA, and a CoA-S-acetyl-ARYFRR bisubstrate inhibitor. The structures are consistent with a direct nucleophilic addition-elimination mechanism with Glu103 and Tyr115 acting as the catalytic base and acid, respectively. The RimI(ST)-bisubstrate complex suggests that several residues change conformation upon interacting with the N terminus of S18, including Glu103, the proposed active site base, facilitating proton exchange and catalysis.
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===RIMI - RIBOSOMAL S18 N-ALPHA-PROTEIN ACETYLTRANSFERASE IN COMPLEX WITH A BISUBSTRATE INHIBITOR (CTERM-ARG-ARG-PHE-TYR-ARG-ALA-N-ALPHA-ACETYL-S-COA).===
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Crystal structure of RimI from Salmonella typhimurium LT2, the GNAT responsible for N(alpha)-acetylation of ribosomal protein S18.,Vetting MW, Bareich DC, Yu M, Blanchard JS Protein Sci. 2008 Oct;17(10):1781-90. Epub 2008 Jul 2. PMID:18596200<ref>PMID:18596200</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18596200}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2cnm" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18596200 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18596200}}
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==About this Structure==
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[[2cnm]] is a 6 chain structure of [[Ribosomal protein S18]] with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium_str._lt2 Salmonella enterica subsp. enterica serovar typhimurium str. lt2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CNM OCA].
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==See Also==
==See Also==
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*[[Ribosomal protein S18]]
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*[[Ribosomal protein S18|Ribosomal protein S18]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:018596200</ref><references group="xtra"/>
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__TOC__
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[[Category: Ribosomal-protein-alanine N-acetyltransferase]]
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</StructureSection>
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[[Category: Salmonella enterica subsp. enterica serovar typhimurium str. lt2]]
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[[Category: Large Structures]]
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[[Category: Bareich, D C.]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]]
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[[Category: Blanchard, J S.]]
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[[Category: Bareich DC]]
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[[Category: Vetting, M W.]]
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[[Category: Blanchard JS]]
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[[Category: Yu, M.]]
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[[Category: Vetting MW]]
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[[Category: Acetyltransferase]]
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[[Category: Yu M]]
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[[Category: Acyltransferase]]
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[[Category: Gcn5-n-acetyltransferase]]
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[[Category: Gnat]]
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[[Category: N-alpha acetylation]]
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[[Category: Ribosomal protein]]
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[[Category: Transferase]]
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Current revision

RimI - Ribosomal S18 N-alpha-protein acetyltransferase in complex with a bisubstrate inhibitor (Cterm-Arg-Arg-Phe-Tyr-Arg-Ala-N-alpha- acetyl-S-CoA).

PDB ID 2cnm

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OCA

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