5bna

[[Image:5bna.jpg|left|200px]]<br /><applet load="5bna" size="350" color="white" frame="true" align="right" spinBox="true"

caption="5bna, resolution 2.600&Aring;" />

'''THE PRIMARY MODE OF BINDING OF CISPLATIN TO A B-DNA DODECAMER: C-G-C-G-A-A-T-T-C-G-C-G'''<br />

==Overview==

When cisplatin [cis- diamminodichloroplatinum (II)] is diffused into, pre-grown crystals of the B-DNA double-helical dodecamer, C-G-C-G-A-A-T-T-C-G-C-G, it binds preferentially to the N7 positions of, guanines, with what probably is an aquo bridge between Pt and the adjacent, O6 atom of the same guanine. The entire guanine ring moves slightly toward, the platinum site, into the major groove. Only three of the eight, potential cisplatin binding sites on guanines actually are occupied, and, this differential reactivity can be explained in terms of the relative, freedom of motion of guanines toward the major groove. This shift of, guanines upon ligation may weaken the glycosyl bond and assist in the, depurination that leads to mismatch SOS repair and G.C. to T.A., transversion.

 

==About this Structure==

5BNA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=PTN:'>PTN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BNA OCA].

 

The primary mode of binding of cisplatin to a B-DNA dodecamer: C-G-C-G-A-A-T-T-C-G-C-G., Wing RM, Pjura P, Drew HR, Dickerson RE, EMBO J. 1984 May;3(5):1201-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=6539674 6539674]

[[Category: Dickerson, R.E.]]

[[Category: Drew, H.R.]]

[[Category: Pjura, P.]]

[[Category: Wing, R.M.]]

[[Category: PTN]]

[[Category: b-dna]]

[[Category: complexed with drug]]

[[Category: double helix]]

[[Category: modified]]