8drh

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(New page: 200px<br /><applet load="8drh" size="350" color="white" frame="true" align="right" spinBox="true" caption="8drh" /> '''HIGH RESOLUTION NMR STRUCTURE OF THE D(GCGTC...)
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[[Image:8drh.jpg|left|200px]]<br /><applet load="8drh" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="8drh" />
 
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'''HIGH RESOLUTION NMR STRUCTURE OF THE D(GCGTCAGG)R(CCUGACGC) HYBRID, MINIMIZED AVERAGE STRUCTURE'''<br />
 
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==Overview==
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==HIGH RESOLUTION NMR STRUCTURE OF THE D(GCGTCAGG)R(CCUGACGC) HYBRID, MINIMIZED AVERAGE STRUCTURE==
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In this work, we present the first NMR solution structure of a DNA/RNA, hybrid containing stereoregular Rp-phosphorothioate modifications of all, DNA backbone linkages. The complex of the enzymatically synthesized, phosphorothioate DNA octamer (all-Rp)-d(GCGTCAGG) and its complementary, RNA r(CCUGACGC) was found to adopt an overall conformation within the, A-form family. Most helical parameters and the sugar puckers of the DNA, strand assume values intermediate between A- and B-form. The close, structural similarity with the unmodified DNA/RNA hybrid of the same, sequence may explain why both the natural and the sulfur-substituted, complex can be recognized and digested by ribonuclease H.
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<StructureSection load='8drh' size='340' side='right'caption='[[8drh]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8drh]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DRH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DRH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8drh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8drh OCA], [https://pdbe.org/8drh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8drh RCSB], [https://www.ebi.ac.uk/pdbsum/8drh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8drh ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In this work, we present the first NMR solution structure of a DNA/RNA hybrid containing stereoregular Rp-phosphorothioate modifications of all DNA backbone linkages. The complex of the enzymatically synthesized phosphorothioate DNA octamer (all-Rp)-d(GCGTCAGG) and its complementary RNA r(CCUGACGC) was found to adopt an overall conformation within the A-form family. Most helical parameters and the sugar puckers of the DNA strand assume values intermediate between A- and B-form. The close structural similarity with the unmodified DNA/RNA hybrid of the same sequence may explain why both the natural and the sulfur-substituted complex can be recognized and digested by ribonuclease H.
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==About this Structure==
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Structure of a stereoregular phosphorothioate DNA/RNA duplex.,Bachelin M, Hessler G, Kurz G, Hacia JG, Dervan PB, Kessler H Nat Struct Biol. 1998 Apr;5(4):271-6. PMID:9546216<ref>PMID:9546216</ref>
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8DRH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DRH OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of a stereoregular phosphorothioate DNA/RNA duplex., Bachelin M, Hessler G, Kurz G, Hacia JG, Dervan PB, Kessler H, Nat Struct Biol. 1998 Apr;5(4):271-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9546216 9546216]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 8drh" style="background-color:#fffaf0;"></div>
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[[Category: Bachelin, M.]]
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== References ==
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[[Category: Dervan, P.B.]]
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<references/>
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[[Category: Hacia, J.G.]]
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__TOC__
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[[Category: Hessler, G.]]
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</StructureSection>
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[[Category: Kessler, H.]]
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[[Category: Large Structures]]
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[[Category: Kurz, G.]]
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[[Category: Bachelin M]]
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[[Category: deoxyribonucleic acid/ribonucleic acid]]
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[[Category: Dervan PB]]
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[[Category: dna/rna hybrid]]
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[[Category: Hacia JG]]
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[[Category: oligonucleotide]]
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[[Category: Hessler G]]
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[[Category: Kessler H]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jan 29 21:52:27 2008''
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[[Category: Kurz G]]

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HIGH RESOLUTION NMR STRUCTURE OF THE D(GCGTCAGG)R(CCUGACGC) HYBRID, MINIMIZED AVERAGE STRUCTURE

PDB ID 8drh

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