2vcb

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[[Image:2vcb.png|left|200px]]
 
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==Family 89 Glycoside Hydrolase from Clostridium perfringens in complex with PUGNAc==
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The line below this paragraph, containing "STRUCTURE_2vcb", creates the "Structure Box" on the page.
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<StructureSection load='2vcb' size='340' side='right'caption='[[2vcb]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2vcb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VCB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VCB FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=OAN:O-(2-ACETAMIDO-2-DEOXY+D-GLUCOPYRANOSYLIDENE)+AMINO-N-PHENYLCARBAMATE'>OAN</scene></td></tr>
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{{STRUCTURE_2vcb| PDB=2vcb | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vcb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vcb OCA], [https://pdbe.org/2vcb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vcb RCSB], [https://www.ebi.ac.uk/pdbsum/2vcb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vcb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A0A0H2YU91_CLOP1 A0A0H2YU91_CLOP1]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vc/2vcb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vcb ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Mucopolysaccharidosis III (MPS III) has four forms (A-D) that result from buildup of an improperly degraded glycosaminoglycan in lysosomes. MPS IIIB is attributable to the decreased activity of a lysosomal alpha-N-acetylglucosaminidase (NAGLU). Here, we describe the structure, catalytic mechanism, and inhibition of CpGH89 from Clostridium perfringens, a close bacterial homolog of NAGLU. The structure enables the generation of a homology model of NAGLU, an enzyme that has resisted structural studies despite having been studied for &gt;20 years. This model reveals which mutations giving rise to MPS IIIB map to the active site and which map to regions distant from the active site. The identification of potent inhibitors of CpGH89 and the structures of these inhibitors in complex with the enzyme suggest small-molecule candidates for use as chemical chaperones. These studies therefore illuminate the genetic basis of MPS IIIB, provide a clear biochemical rationale for the necessary sequential action of heparan-degrading enzymes, and open the door to the design and optimization of chemical chaperones for treating MPS IIIB.
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===FAMILY 89 GLYCOSIDE HYDROLASE FROM CLOSTRIDIUM PERFRINGENS IN COMPLEX WITH PUGNAC===
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Structural and mechanistic insight into the basis of mucopolysaccharidosis IIIB.,Ficko-Blean E, Stubbs KA, Nemirovsky O, Vocadlo DJ, Boraston AB Proc Natl Acad Sci U S A. 2008 May 6;105(18):6560-5. Epub 2008 Apr 28. PMID:18443291<ref>PMID:18443291</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_18443291}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2vcb" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 18443291 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18443291}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[2vcb]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clostridium_perfringens Clostridium perfringens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VCB OCA].
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==Reference==
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<ref group="xtra">PMID:018443291</ref><references group="xtra"/>
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[[Category: Alpha-N-acetylglucosaminidase]]
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[[Category: Clostridium perfringens]]
[[Category: Clostridium perfringens]]
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[[Category: Berg, O.]]
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[[Category: Large Structures]]
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[[Category: Boraston, A B.]]
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[[Category: Berg O]]
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[[Category: Ficko-Blean, E.]]
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[[Category: Boraston AB]]
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[[Category: Stubbs, K A.]]
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[[Category: Ficko-Blean E]]
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[[Category: Vocadlo, D J.]]
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[[Category: Stubbs KA]]
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[[Category: Alpha-n-acetylglucosaminidase]]
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[[Category: Vocadlo DJ]]
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[[Category: Family 89 glycoside hydrolase]]
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[[Category: Gh89]]
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[[Category: Hydrolase]]
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[[Category: Mucopolysaccharidosis]]
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[[Category: Naglu]]
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[[Category: Pugnac]]
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[[Category: Sanfilippo disease]]
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Current revision

Family 89 Glycoside Hydrolase from Clostridium perfringens in complex with PUGNAc

PDB ID 2vcb

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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