1w0d

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[[Image:1w0d.png|left|200px]]
 
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==The high resolution structure of Mycobacterium tuberculosis LeuB (Rv2995c)==
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The line below this paragraph, containing "STRUCTURE_1w0d", creates the "Structure Box" on the page.
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<StructureSection load='1w0d' size='340' side='right'caption='[[1w0d]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1w0d]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W0D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W0D FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1w0d| PDB=1w0d | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w0d OCA], [https://pdbe.org/1w0d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w0d RCSB], [https://www.ebi.ac.uk/pdbsum/1w0d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w0d ProSAT], [https://www.topsan.org/Proteins/XMTB/1w0d TOPSAN]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LEU3_MYCTU LEU3_MYCTU] Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.[HAMAP-Rule:MF_01035]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w0/1w0d_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1w0d ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of the enzyme 3-isopropylmalate dehydrogenase (IPMDH) from Mycobacterium tuberculosis (LeuB, Mtb-IPMDH, Rv2995c) without substrate or co-factor was determined at 1.65 A resolution, which is the highest resolution reported for an IPMDH to date. The crystals contain two functional dimers in the asymmetric unit in an arrangement close to a tetramer of D2 symmetry. Despite the absence of a substrate or inhibitor bound to the protein, the structure of the monomer resembles the previously observed closed form of the enzyme more closely than the open form. A comparison with the substrate complex of IPMDH from Thiobacillus ferrooxidans and the co-factor complex of the Thermus thermophilus enzyme revealed a close relationship of the active-site architecture between the various bacterial enzymes. The inhibitor O-isobutenyl oxalylhydroxamate was found to bind to the active site of IPMDH in a mode similar to the substrate isopropylmalate.
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===THE HIGH RESOLUTION STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS LEUB (RV2995C)===
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The high-resolution Structure of LeuB (Rv2995c) from Mycobacterium tuberculosis.,Singh RK, Kefala G, Janowski R, Mueller-Dieckmann C, von Kries JP, Weiss MS J Mol Biol. 2005 Feb 11;346(1):1-11. Epub 2004 Dec 23. PMID:15663922<ref>PMID:15663922</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1w0d" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_15663922}}, adds the Publication Abstract to the page
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*[[Isopropylmalate dehydrogenase|Isopropylmalate dehydrogenase]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15663922 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15663922}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[1w0d]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W0D OCA].
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Janowski R]]
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==Reference==
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[[Category: Kefala G]]
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<ref group="xtra">PMID:015663922</ref><references group="xtra"/>
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[[Category: Mueller-Dieckmann C]]
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[[Category: 3-isopropylmalate dehydrogenase]]
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[[Category: Singh RK]]
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Weiss MS]]
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[[Category: Janowski, R.]]
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[[Category: Kefala, G.]]
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[[Category: Mueller-Dieckmann, C.]]
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[[Category: Singh, R K.]]
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[[Category: Tb , Tb Structural Genomics Consortium]]
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[[Category: Weiss, M S.]]
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[[Category: Dehydrogenase]]
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[[Category: Leucine biosynthesis]]
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[[Category: Nad]]
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[[Category: Oxidoreductase]]
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[[Category: Protein structure initiative]]
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[[Category: Psi]]
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[[Category: Structural genomic]]
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[[Category: Tb]]
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[[Category: Tb structural genomics consortium]]
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[[Category: Tbsgc]]
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Current revision

The high resolution structure of Mycobacterium tuberculosis LeuB (Rv2995c)

PDB ID 1w0d

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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