2jon

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(New page: 200px<br /><applet load="2jon" size="350" color="white" frame="true" align="right" spinBox="true" caption="2jon" /> '''Solution structure of the C-terminal domain ...)
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[[Image:2jon.jpg|left|200px]]<br /><applet load="2jon" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2jon" />
 
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'''Solution structure of the C-terminal domain Ole e 9'''<br />
 
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==Overview==
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==Solution structure of the C-terminal domain Ole e 9==
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Ole e 9 is an olive pollen allergen belonging to group 2 of, pathogenesis-related proteins. The protein is composed of two, immunological independent domains: an N-terminal domain (NtD) with, 1,3-beta-glucanase activity, and a C-terminal domain (CtD) that binds, 1,3-beta-glucans. We have determined the three-dimensional structure of, CtD-Ole e 9 (101 amino acids), which consists of two parallel, alpha-helices forming an angle of approximately 55 degrees , a small, antiparallel beta-sheet with two short strands, and a 3-10 helix turn, all, connected by long coil segments, resembling a novel type of folding among, allergens. Two regions surrounded by aromatic residues (F49, Y60, F96, Y91, and Y31, H68, Y65, F78) have been localized on the protein surface, and a, role for sugar binding is suggested. The epitope mapping of CtD-Ole e 9, shows that B-cell epitopes are mainly located on loops, although some of, them are contained in secondary structural elements. Interestingly, the, IgG and IgE epitopes are contiguous or overlapped, rather than coincident., The three-dimensional structure of CtD-Ole e 9 might help to understand, the underlying mechanism of its biochemical function and to determine, possible structure-allergenicity relationships.
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<StructureSection load='2jon' size='340' side='right'caption='[[2jon]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2jon]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Olea_europaea Olea europaea]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JON OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JON FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jon OCA], [https://pdbe.org/2jon PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jon RCSB], [https://www.ebi.ac.uk/pdbsum/2jon PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jon ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ALL9_OLEEU ALL9_OLEEU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jo/2jon_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jon ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Ole e 9 is an olive pollen allergen belonging to group 2 of pathogenesis-related proteins. The protein is composed of two immunological independent domains: an N-terminal domain (NtD) with 1,3-beta-glucanase activity, and a C-terminal domain (CtD) that binds 1,3-beta-glucans. We have determined the three-dimensional structure of CtD-Ole e 9 (101 amino acids), which consists of two parallel alpha-helices forming an angle of approximately 55 degrees , a small antiparallel beta-sheet with two short strands, and a 3-10 helix turn, all connected by long coil segments, resembling a novel type of folding among allergens. Two regions surrounded by aromatic residues (F49, Y60, F96, Y91 and Y31, H68, Y65, F78) have been localized on the protein surface, and a role for sugar binding is suggested. The epitope mapping of CtD-Ole e 9 shows that B-cell epitopes are mainly located on loops, although some of them are contained in secondary structural elements. Interestingly, the IgG and IgE epitopes are contiguous or overlapped, rather than coincident. The three-dimensional structure of CtD-Ole e 9 might help to understand the underlying mechanism of its biochemical function and to determine possible structure-allergenicity relationships.
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==About this Structure==
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Solution structure of the C-terminal domain of Ole e 9, a major allergen of olive pollen.,Trevino MA, Palomares O, Castrillo I, Villalba M, Rodriguez R, Rico M, Santoro J, Bruix M Protein Sci. 2008 Feb;17(2):371-6. Epub 2007 Dec 20. PMID:18096638<ref>PMID:18096638</ref>
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2JON is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Olea_europaea Olea europaea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JON OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Solution structure of the C-terminal domain of Ole e 9, a major allergen of olive pollen., Trevino MA, Palomares O, Castrillo I, Villalba M, Rodriguez R, Rico M, Santoro J, Bruix M, Protein Sci. 2008 Feb;17(2):371-6. Epub 2007 Dec 20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18096638 18096638]
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</div>
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[[Category: Olea europaea]]
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<div class="pdbe-citations 2jon" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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[[Category: Bruix, M.]]
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[[Category: Castrillo, I.]]
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[[Category: Palomares, O.]]
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[[Category: Rico, M.]]
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[[Category: Rodriguez, R.]]
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[[Category: Santoro, J.]]
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[[Category: Trevino, M.A.]]
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[[Category: Villalba, M.]]
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[[Category: allergen]]
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[[Category: olive pollen]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 31 10:57:20 2008''
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==See Also==
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*[[Glucanase 3D structures|Glucanase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Olea europaea]]
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[[Category: Bruix M]]
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[[Category: Castrillo I]]
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[[Category: Palomares O]]
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[[Category: Rico M]]
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[[Category: Rodriguez R]]
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[[Category: Santoro J]]
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[[Category: Trevino MA]]
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[[Category: Villalba M]]

Current revision

Solution structure of the C-terminal domain Ole e 9

PDB ID 2jon

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