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2pbi

From Proteopedia

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(New page: 200px<br /><applet load="2pbi" size="350" color="white" frame="true" align="right" spinBox="true" caption="2pbi, resolution 1.950&Aring;" /> '''The multifunctional...)
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[[Image:2pbi.jpg|left|200px]]<br /><applet load="2pbi" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2pbi, resolution 1.950&Aring;" />
 
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'''The multifunctional nature of Gbeta5/RGS9 revealed from its crystal structure'''<br />
 
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==Overview==
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==The multifunctional nature of Gbeta5/RGS9 revealed from its crystal structure==
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Regulators of G-protein signaling (RGS) proteins enhance the intrinsic, GTPase activity of G protein alpha (Galpha) subunits and are vital for, proper signaling kinetics downstream of G protein-coupled receptors, (GPCRs). R7 subfamily RGS proteins specifically and obligately dimerize, with the atypical G protein beta5 (Gbeta5) subunit through an internal G, protein gamma (Ggamma)-subunit-like (GGL) domain. Here we present the, 1.95-A crystal structure of the Gbeta5-RGS9 complex, which is essential, for normal visual and neuronal signal transduction. This structure reveals, a canonical RGS domain that is functionally integrated within a molecular, complex that is poised for integration of multiple steps during G-protein, activation and deactivation.
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<StructureSection load='2pbi' size='340' side='right'caption='[[2pbi]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2pbi]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PBI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PBI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pbi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pbi OCA], [https://pdbe.org/2pbi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pbi RCSB], [https://www.ebi.ac.uk/pdbsum/2pbi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pbi ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RGS9_MOUSE RGS9_MOUSE] Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G(t)-alpha. Involved in phototransduction; key element in the recovery phase of visual transduction.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pb/2pbi_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pbi ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2PBI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Sites: <scene name='pdbsite=AC1:Gol Binding Site For Residue D 901'>AC1</scene>, <scene name='pdbsite=AC2:Gol Binding Site For Residue B 902'>AC2</scene>, <scene name='pdbsite=AC3:Gol Binding Site For Residue D 903'>AC3</scene>, <scene name='pdbsite=AC4:Gol Binding Site For Residue A 904'>AC4</scene>, <scene name='pdbsite=AC5:Gol Binding Site For Residue A 905'>AC5</scene>, <scene name='pdbsite=AC6:Gol Binding Site For Residue A 906'>AC6</scene>, <scene name='pdbsite=AC7:Gol Binding Site For Residue C 907'>AC7</scene>, <scene name='pdbsite=AC8:Gol Binding Site For Residue D 908'>AC8</scene> and <scene name='pdbsite=AC9:Gol Binding Site For Residue B 909'>AC9</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PBI OCA].
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*[[Regulator of G-protein signaling 3D structures|Regulator of G-protein signaling 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of the multifunctional Gbeta5-RGS9 complex., Cheever ML, Snyder JT, Gershburg S, Siderovski DP, Harden TK, Sondek J, Nat Struct Mol Biol. 2008 Jan 20;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18204463 18204463]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Cheever ML]]
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[[Category: Cheever, M.L.]]
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[[Category: Gershburg S]]
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[[Category: Gershburg, S.]]
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[[Category: Harden TK]]
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[[Category: Harden, T.K.]]
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[[Category: Siderovski DP]]
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[[Category: Siderovski, D.P.]]
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[[Category: Snyder JT]]
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[[Category: Snyder, J.T.]]
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[[Category: Sondek J]]
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[[Category: Sondek, J.]]
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[[Category: GOL]]
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[[Category: beta propeller]]
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[[Category: dep domain]]
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[[Category: dhex domain]]
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[[Category: ggl domain]]
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[[Category: helix wrap]]
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[[Category: rgs domain]]
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[[Category: signaling protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 31 10:58:32 2008''
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Current revision

The multifunctional nature of Gbeta5/RGS9 revealed from its crystal structure

PDB ID 2pbi

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