2r15

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(New page: 200px<br /><applet load="2r15" size="350" color="white" frame="true" align="right" spinBox="true" caption="2r15, resolution 2.24&Aring;" /> '''Crystal structure of...)
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[[Image:2r15.jpg|left|200px]]<br /><applet load="2r15" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2r15, resolution 2.24&Aring;" />
 
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'''Crystal structure of the myomesin domains 12 and 13'''<br />
 
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==Overview==
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==Crystal structure of the myomesin domains 12 and 13==
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Sarcomeric filament proteins display extraordinary properties in terms of, protein length and mechanical elasticity, requiring specific anchoring and, assembly mechanisms. To establish the molecular basis of terminal filament, assembly, we have selected the sarcomeric M-band protein myomesin as a, prototypic filament model. The crystal structure of the myomesin, C-terminus, comprising a tandem array of two immunoglobulin (Ig) domains, My12 and My13, reveals a dimeric end-to-end filament of 14.3 nm length., Although the two domains share the same fold, an unexpected rearrangement, of one beta-strand reveals how they are evolved into unrelated functions, terminal filament assembly (My13) and filament propagation (My12). The two, domains are connected by a six-turn alpha-helix, of which two turns are, void of any interactions with other protein parts. Thus, the overall, structure of the assembled myomesin C-terminus resembles a three-body, beads-on-the-string model with potentially elastic properties. We predict, that the found My12-helix-My13 domain topology may provide a structural, template for the filament architecture of the entire C-terminal Ig domain, array My9-My13 of myomesin.
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<StructureSection load='2r15' size='340' side='right'caption='[[2r15]], [[Resolution|resolution]] 2.24&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2r15]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R15 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.24&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r15 OCA], [https://pdbe.org/2r15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r15 RCSB], [https://www.ebi.ac.uk/pdbsum/2r15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r15 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MYOM1_HUMAN MYOM1_HUMAN] Major component of the vertebrate myofibrillar M band. Binds myosin, titin, and light meromyosin. This binding is dose dependent.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r1/2r15_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r15 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2R15 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ACT:'>ACT</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:Gol Binding Site For Residue A 601'>AC1</scene>, <scene name='pdbsite=AC2:Gol Binding Site For Residue B 602'>AC2</scene> and <scene name='pdbsite=AC3:Gol Binding Site For Residue B 603'>AC3</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R15 OCA].
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*[[Myomesin|Myomesin]]
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__TOC__
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==Reference==
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</StructureSection>
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Molecular basis of the C-terminal tail-to-tail assembly of the sarcomeric filament protein myomesin., Pinotsis N, Lange S, Perriard JC, Svergun DI, Wilmanns M, EMBO J. 2008 Jan 9;27(1):253-64. Epub 2007 Dec 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18059477 18059477]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Lange, S.]]
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[[Category: Lange S]]
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[[Category: Pinotsis, N.]]
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[[Category: Pinotsis N]]
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[[Category: Wilmanns, M.]]
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[[Category: Wilmanns M]]
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[[Category: ACT]]
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[[Category: GOL]]
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[[Category: contractile protein]]
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[[Category: homodimer]]
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[[Category: ig-like domains]]
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[[Category: immunoglobulin domain]]
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[[Category: muscle protein]]
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[[Category: sarcomeric protein]]
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[[Category: thick filament]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jan 31 10:59:27 2008''
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Current revision

Crystal structure of the myomesin domains 12 and 13

PDB ID 2r15

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