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1h2f

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[[Image:1h2f.gif|left|200px]]<br />
 
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<applet load="1h2f" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1h2f, resolution 2.00&Aring;" />
 
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'''BACILLUS STEAROTHERMOPHILUS PHOE (PREVIOUSLY KNOWN AS YHFR) IN COMPLEX WITH TRIVANADATE'''<br />
 
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==Overview==
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==BACILLUS STEAROTHERMOPHILUS PHOE (previously known as yhfr) in complex with trivanadate==
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Bacillus stearothermophilus phosphatase PhoE is a member of the, cofactor-dependent phosphoglycerate mutase superfamily possessing broad, specificity phosphatase activity. Its previous structural determination in, complex with glycerol revealed probable bases for its efficient hydrolysis, of both large, hydrophobic, and smaller, hydrophilic substrates. Here we, report two further structures of PhoE complexes, to higher resolution of, diffraction, which yield a better and thorough understanding of its, catalytic mechanism. The environment of the phosphate ion in the catalytic, site of the first complex strongly suggests an acid-base catalytic, function for Glu83. It also reveals how the C-terminal tail ordering is, linked to enzyme activation on phosphate binding by a different mechanism, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?12498792 (full description)]]
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<StructureSection load='1h2f' size='340' side='right'caption='[[1h2f]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1h2f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H2F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H2F FirstGlance]. <br>
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1H2F is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]] with PO4 and VA3 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: VA3. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H2F OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=VA3:TRIVANADATE'>VA3</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h2f OCA], [https://pdbe.org/1h2f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h2f RCSB], [https://www.ebi.ac.uk/pdbsum/1h2f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h2f ProSAT]</span></td></tr>
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Structures of phosphate and trivanadate complexes of Bacillus stearothermophilus phosphatase PhoE: structural and functional analysis in the cofactor-dependent phosphoglycerate mutase superfamily., Rigden DJ, Littlejohn JE, Henderson K, Jedrzejas MJ, J Mol Biol. 2003 Jan 17;325(3):411-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12498792 12498792]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9ALU0_GEOSE Q9ALU0_GEOSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h2/1h2f_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h2f ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Geobacillus stearothermophilus]]
[[Category: Geobacillus stearothermophilus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Jedrzejas, M.J.]]
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[[Category: Jedrzejas MJ]]
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[[Category: Littlejohn, J.]]
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[[Category: Littlejohn JE]]
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[[Category: Rigden, D.J.]]
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[[Category: Rigden DJ]]
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[[Category: PO4]]
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[[Category: VA3]]
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[[Category: broad specificity phosphatase; dpgm homolog]]
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[[Category: hydrolase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:24:52 2007''
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Current revision

BACILLUS STEAROTHERMOPHILUS PHOE (previously known as yhfr) in complex with trivanadate

PDB ID 1h2f

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