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1ll5

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[[Image:1ll5.png|left|200px]]
 
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==X-ray crystal structure of AmpC WT beta-lactamase in complex with covalently bound imipenem==
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The line below this paragraph, containing "STRUCTURE_1ll5", creates the "Structure Box" on the page.
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<StructureSection load='1ll5' size='340' side='right'caption='[[1ll5]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1ll5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LL5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LL5 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IM2:(5R)-5-[(1S,2R)-1-FORMYL-2-HYDROXYPROPYL]-3-[(2-{[(E)-IMINOMETHYL]AMINO}ETHYL)SULFANYL]-4,5-DIHYDRO-1H-PYRROLE-2-CARBOXYLIC+ACID'>IM2</scene></td></tr>
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{{STRUCTURE_1ll5| PDB=1ll5 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ll5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ll5 OCA], [https://pdbe.org/1ll5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ll5 RCSB], [https://www.ebi.ac.uk/pdbsum/1ll5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ll5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AMPC_ECOLI AMPC_ECOLI] This protein is a serine beta-lactamase with a substrate specificity for cephalosporins.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ll/1ll5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ll5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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To determine how imipenem inhibits the class C beta-lactamase AmpC, the X-ray crystal structure of the acyl-enzyme complex was determined to a resolution of 1.80 A. In the complex, the lactam carbonyl oxygen of imipenem has flipped by approximately 180 degrees compared to its expected position; the electrophilic acyl center is thus displaced from the point of hydrolytic attack. This conformation resembles that of imipenem bound to the class A enzyme TEM-1 but is different from that of moxalactam bound to AmpC.
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===X-ray crystal structure of AmpC WT beta-lactamase in complex with covalently bound imipenem===
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Structural basis for imipenem inhibition of class C beta-lactamases.,Beadle BM, Shoichet BK Antimicrob Agents Chemother. 2002 Dec;46(12):3978-80. PMID:12435704<ref>PMID:12435704</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1ll5" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_12435704}}, adds the Publication Abstract to the page
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*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 12435704 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12435704}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[1ll5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LL5 OCA].
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==Reference==
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<ref group="xtra">PMID:012435704</ref><references group="xtra"/>
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[[Category: Beta-lactamase]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Beadle, B M.]]
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[[Category: Large Structures]]
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[[Category: Shoichet, B K.]]
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[[Category: Beadle BM]]
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[[Category: Beta-lactamase]]
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[[Category: Shoichet BK]]
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[[Category: Carbapenem]]
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[[Category: Hydrolase]]
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[[Category: Imipenem]]
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Current revision

X-ray crystal structure of AmpC WT beta-lactamase in complex with covalently bound imipenem

PDB ID 1ll5

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