3p9o

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[[Image:3p9o.jpg|left|200px]]
 
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==Aerobic ternary complex of urate oxidase with azide and chloride==
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The line below this paragraph, containing "STRUCTURE_3p9o", creates the "Structure Box" on the page.
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<StructureSection load='3p9o' size='340' side='right'caption='[[3p9o]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3p9o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_flavus Aspergillus flavus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P9O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P9O FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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{{STRUCTURE_3p9o| PDB=3p9o | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p9o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p9o OCA], [https://pdbe.org/3p9o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p9o RCSB], [https://www.ebi.ac.uk/pdbsum/3p9o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p9o ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/URIC_ASPFL URIC_ASPFL] Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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BACKGROUND: Urate oxidase (EC 1.7.3.3 or UOX) catalyzes the conversion of uric acid and gaseous molecular oxygen to 5-hydroxyisourate and hydrogen peroxide, in the absence of cofactor or particular metal cation. The functional enzyme is a homo-tetramer with four active sites located at dimeric interfaces. RESULTS: The catalytic mechanism was investigated through a ternary complex formed between the enzyme, uric acid, and cyanide that stabilizes an intermediate state of the reaction. When uric acid is replaced by a competitive inhibitor, no complex with cyanide is formed. CONCLUSION: The X-ray structure of this compulsory ternary complex led to a number of mechanistic evidences that support a sequential mechanism in which the two reagents, dioxygen and a water molecule, process through a common site located 3.3 A above the mean plane of the ligand. This site is built by the side chains of Asn 254, and Thr 57, two conserved residues belonging to two different subunits of the homo-tetramer. The absence of a ternary complex between the enzyme, a competitive inhibitor, and cyanide suggests that cyanide inhibits the hydroxylation step of the reaction, after the initial formation of a hydroperoxyde type intermediate.
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===Aerobic ternary complex of urate oxidase with azide and chloride===
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Structural analysis of urate oxidase in complex with its natural substrate inhibited by cyanide: mechanistic implications.,Gabison L, Prange T, Colloc'h N, El Hajji M, Castro B, Chiadmi M BMC Struct Biol. 2008 Jul 20;8:32. PMID:018638417<ref>PMID:018638417</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3p9o" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18638417}}, adds the Publication Abstract to the page
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*[[Urate oxidase 3D structures|Urate oxidase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18638417 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18638417}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[3p9o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_flavus Aspergillus flavus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P9O OCA].
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==Reference==
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<ref group="xtra">PMID:018638417</ref><references group="xtra"/>
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[[Category: Aspergillus flavus]]
[[Category: Aspergillus flavus]]
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[[Category: Urate oxidase]]
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[[Category: Large Structures]]
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[[Category: Castro, B.]]
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[[Category: Castro B]]
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[[Category: Chiadmi, M.]]
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[[Category: Chiadmi M]]
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[[Category: Gabison, L.]]
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[[Category: Colloc'H N]]
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[[Category: H, N Colloc.]]
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[[Category: El Hajji M]]
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[[Category: Hajji, M El.]]
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[[Category: Gabison L]]
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[[Category: Prange, T.]]
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[[Category: Prange T]]
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[[Category: Azide]]
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[[Category: Degradation mechanism]]
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[[Category: Inhibition]]
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[[Category: Oxygen binding]]
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[[Category: Oxygen binding oxidoreductase]]
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[[Category: Peroxisome]]
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[[Category: Purine metabolism]]
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[[Category: Uric acid]]
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Current revision

Aerobic ternary complex of urate oxidase with azide and chloride

PDB ID 3p9o

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