3hu7

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[[Image:3hu7.png|left|200px]]
 
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==Structural characterization and binding studies of a plant pathogenesis related protein heamanthin from haemanthus multiflorus reveal its dual inhibitory effects against xylanase and alpha-amylase==
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The line below this paragraph, containing "STRUCTURE_3hu7", creates the "Structure Box" on the page.
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<StructureSection load='3hu7' size='340' side='right'caption='[[3hu7]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3hu7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Scadoxus_multiflorus Scadoxus multiflorus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HU7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HU7 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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{{STRUCTURE_3hu7| PDB=3hu7 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hu7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hu7 OCA], [https://pdbe.org/3hu7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hu7 RCSB], [https://www.ebi.ac.uk/pdbsum/3hu7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hu7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/B2ZGS7_9ASPA B2ZGS7_9ASPA]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hu/3hu7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hu7 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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A novel plant protein isolated from the underground bulbs of Scadoxus multiflorus, xylanase and alpha-amylase inhibitor protein (XAIP), inhibits two structurally and functionally unrelated enzymes: xylanase and alpha-amylase. The mature protein contains 272 amino acid residues which show sequence identities of 48% to the plant chitinase hevamine and 36% to xylanase inhibitor protein-I, a double-headed inhibitor of GH10 and GH11 xylanases. However, unlike hevamine, it is enzymatically inactive and, unlike xylanase inhibitor protein-I, it inhibits two functionally different classes of enzyme. The crystal structure of XAIP has been determined at 2.0 A resolution and refined to R(cryst) and R(free) factors of 15.2% and 18.6%, respectively. The polypeptide chain of XAIP adopts a modified triosephosphate isomerase barrel fold with eight beta-strands in the inner circle and nine alpha-helices forming the outer ring. The structure contains three cis peptide bonds: Gly33-Phe34, Tyr159-Pro160 and Trp253-Asp254. Although hevamine has a long accessible carbohydrate-binding channel, in XAIP this channel is almost completely filled with the side-chains of residues Phe13, Pro77, Lys78 and Trp253. Solution studies indicate that XAIP inhibits GH11 family xylanases and GH13 family alpha-amylases through two independent binding sites located on opposite surfaces of the protein. Comparison of the structure of XAIP with that of xylanase inhibitor protein-I, and docking studies, suggest that loops alpha3-beta4 and alpha4-beta5 may be involved in the binding of GH11 xylanase, and that helix alpha7 and loop beta6-alpha6 are suitable for the interaction with alpha-amylase.
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===Structural characterization and binding studies of a plant pathogenesis related protein heamanthin from haemanthus multiflorus reveal its dual inhibitory effects against xylanase and alpha-amylase===
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Crystal structure determination and inhibition studies of a novel xylanase and alpha-amylase inhibitor protein (XAIP) from Scadoxus multiflorus.,Kumar S, Singh N, Sinha M, Dube D, Singh SB, Bhushan A, Kaur P, Srinivasan A, Sharma S, Singh TP FEBS J. 2010 Jul;277(13):2868-82. Epub 2010 May 27. PMID:20528916<ref>PMID:20528916</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20528916}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3hu7" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20528916 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20528916}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[3hu7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Scadoxus_multiflorus Scadoxus multiflorus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HU7 OCA].
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==Reference==
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<ref group="xtra">PMID:020528916</ref><references group="xtra"/>
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[[Category: Scadoxus multiflorus]]
[[Category: Scadoxus multiflorus]]
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[[Category: Bhushan, A.]]
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[[Category: Bhushan A]]
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[[Category: Kaur, P.]]
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[[Category: Kaur P]]
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[[Category: Kumar, S.]]
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[[Category: Kumar S]]
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[[Category: Sharma, S.]]
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[[Category: Sharma S]]
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[[Category: Singh, N.]]
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[[Category: Singh N]]
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[[Category: Singh, T P.]]
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[[Category: Singh TP]]
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[[Category: Sinha, M.]]
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[[Category: Sinha M]]
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[[Category: Srinivasan, A.]]
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[[Category: Srinivasan A]]
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[[Category: Inhibitor]]
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[[Category: Plant pathogenesis related protein]]
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[[Category: Protein binding]]
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[[Category: Tim barrel]]
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Current revision

Structural characterization and binding studies of a plant pathogenesis related protein heamanthin from haemanthus multiflorus reveal its dual inhibitory effects against xylanase and alpha-amylase

PDB ID 3hu7

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