1aqp

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[[Image:1aqp.jpg|left|200px]]<br /><applet load="1aqp" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1aqp, resolution 2.0&Aring;" />
 
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'''RIBONUCLEASE A COPPER COMPLEX'''<br />
 
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==Overview==
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==RIBONUCLEASE A COPPER COMPLEX==
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We report the crystal structures of the copper and nickel complexes of, RNase A. The overall topology of these two complexes is similar to that of, other RNase A structures. However, there are significant differences in, the mode of binding of copper and nickel. There are two copper ions per, molecule of the protein, but there is only one nickel ion per molecule of, the protein. Significant changes occur in the interprotein interactions as, a result of differences in the coordinating groups at the common binding, site around His-105. Consequently, the copper- and nickel-ion-bound dimers, of RNase A act as nucleation sites for generating different crystal, lattices for the two complexes. A second copper ion is present at an, active site residue His-119 for which all the ligands are from one, molecule of the protein. At this second site, His-119 adopts an inactive, conformation (B) induced by the copper. We have identified a novel copper, binding motif involving the alpha-amino group and the N-terminal residues.
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<StructureSection load='1aqp' size='340' side='right'caption='[[1aqp]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1aqp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AQP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AQP FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aqp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aqp OCA], [https://pdbe.org/1aqp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aqp RCSB], [https://www.ebi.ac.uk/pdbsum/1aqp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aqp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aq/1aqp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1aqp ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We report the crystal structures of the copper and nickel complexes of RNase A. The overall topology of these two complexes is similar to that of other RNase A structures. However, there are significant differences in the mode of binding of copper and nickel. There are two copper ions per molecule of the protein, but there is only one nickel ion per molecule of the protein. Significant changes occur in the interprotein interactions as a result of differences in the coordinating groups at the common binding site around His-105. Consequently, the copper- and nickel-ion-bound dimers of RNase A act as nucleation sites for generating different crystal lattices for the two complexes. A second copper ion is present at an active site residue His-119 for which all the ligands are from one molecule of the protein. At this second site, His-119 adopts an inactive conformation (B) induced by the copper. We have identified a novel copper binding motif involving the alpha-amino group and the N-terminal residues.
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==About this Structure==
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Crystal structures of the copper and nickel complexes of RNase A: metal-induced interprotein interactions and identification of a novel copper binding motif.,Balakrishnan R, Ramasubbu N, Varughese KI, Parthasarathy R Proc Natl Acad Sci U S A. 1997 Sep 2;94(18):9620-5. PMID:9275172<ref>PMID:9275172</ref>
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1AQP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with <scene name='pdbligand=CU:'>CU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Known structural/functional Site: <scene name='pdbsite=CII:Cu+Binding+Site+II'>CII</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AQP OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structures of the copper and nickel complexes of RNase A: metal-induced interprotein interactions and identification of a novel copper binding motif., Balakrishnan R, Ramasubbu N, Varughese KI, Parthasarathy R, Proc Natl Acad Sci U S A. 1997 Sep 2;94(18):9620-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9275172 9275172]
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</div>
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[[Category: Bos taurus]]
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<div class="pdbe-citations 1aqp" style="background-color:#fffaf0;"></div>
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[[Category: Pancreatic ribonuclease]]
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[[Category: Single protein]]
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[[Category: Ramasubbu, N.]]
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[[Category: CU]]
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[[Category: hydrolase (phosphoric diester)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:31:23 2008''
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==See Also==
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Bos taurus]]
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[[Category: Large Structures]]
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[[Category: Ramasubbu N]]

Current revision

RIBONUCLEASE A COPPER COMPLEX

PDB ID 1aqp

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