3aul

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[[Image:3aul.jpg|left|200px]]
 
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==Crystal structure of wild-type Lys48-linked diubiquitin in an open conformation==
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The line below this paragraph, containing "STRUCTURE_3aul", creates the "Structure Box" on the page.
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<StructureSection load='3aul' size='340' side='right'caption='[[3aul]], [[Resolution|resolution]] 2.39&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3aul]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AUL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AUL FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.39&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3aul FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aul OCA], [https://pdbe.org/3aul PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3aul RCSB], [https://www.ebi.ac.uk/pdbsum/3aul PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3aul ProSAT]</span></td></tr>
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{{STRUCTURE_3aul| PDB=3aul | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/UBC_HUMAN UBC_HUMAN] Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.<ref>PMID:16543144</ref> <ref>PMID:19754430</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Proteasomal degradation is mediated through modification of target proteins by Lys-48-linked polyubiquitin (polyUb) chain, which interacts with several binding partners in this pathway through hydrophobic surfaces on individual Ub units. However, the previously reported crystal structures of Lys-48-linked diUb exhibit a closed conformation with sequestered hydrophobic surfaces. NMR studies on mutated Lys-48-linked diUb indicated a pH-dependent conformational equilibrium between closed and open states with the predominance of the former under neutral conditions (90% at pH 6.8). To address the question of how Ub-binding proteins can efficiently access the sequestered hydrophobic surfaces of Ub chains, we revisited the conformational dynamics of Lys-48-linked diUb in solution using wild-type diUb and cyclic forms of diUb in which the Ub units are connected through two Lys-48-mediated isopeptide bonds. Our newly determined crystal structure of wild-type diUb showed an open conformation, whereas NMR analyses of cyclic Lys-48-linked diUb in solution revealed that its structure resembled the closed conformation observed in previous crystal structures. Comparison of a chemical shift of wild-type diUb with that of monomeric Ub and cyclic diUb, which mimic the open and closed states, respectively, with regard to the exposure of hydrophobic surfaces to the solvent indicates that wild-type Lys-48-linked diUb in solution predominantly exhibits the open conformation (75% at pH 7.0), which becomes more populated upon lowering pH. The intrinsic properties of Lys-48-linked Ub chains to adopt the open conformation may be advantageous for interacting with Ub-binding proteins.
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===Crystal structure of wild-type Lys48-linked diubiquitin in an open conformation===
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Conformational Dynamics of Wild-type Lys-48-linked Diubiquitin in Solution.,Hirano T, Serve O, Yagi-Utsumi M, Takemoto E, Hiromoto T, Satoh T, Mizushima T, Kato K J Biol Chem. 2011 Oct 28;286(43):37496-502. Epub 2011 Sep 7. PMID:21900242<ref>PMID:21900242</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3aul" style="background-color:#fffaf0;"></div>
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==About this Structure==
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==See Also==
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[[3aul]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AUL OCA].
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*[[3D structures of ubiquitin|3D structures of ubiquitin]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Hirano, T.]]
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[[Category: Large Structures]]
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[[Category: Hiromoto, T.]]
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[[Category: Hirano T]]
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[[Category: Kato, K.]]
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[[Category: Hiromoto T]]
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[[Category: Mizushima, T.]]
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[[Category: Kato K]]
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[[Category: Olivier, S.]]
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[[Category: Mizushima T]]
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[[Category: Satoh, T.]]
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[[Category: Olivier S]]
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[[Category: Takemoto, E.]]
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[[Category: Satoh T]]
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[[Category: Yagi, M.]]
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[[Category: Takemoto E]]
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[[Category: Lys48-linked diubiquitin]]
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[[Category: Yagi M]]
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[[Category: Signaling protein]]
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[[Category: Ubiquitin]]
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Current revision

Crystal structure of wild-type Lys48-linked diubiquitin in an open conformation

PDB ID 3aul

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