1bb6

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[[Image:1bb6.gif|left|200px]]<br /><applet load="1bb6" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bb6, resolution 2.0&Aring;" />
 
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'''LYSOZYME COMPLEX WITH 4-METHYL-UMBELLIFERYL CHITOTRIOSE'''<br />
 
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==Overview==
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==LYSOZYME COMPLEX WITH 4-METHYL-UMBELLIFERYL CHITOTRIOSE==
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Two complexes between rainbow trout lysozyme (RBTL) and, 4-methylumbelliferyl chitobioside, 4MeU-(GlcNAc)2, and chitotrioside, 4MeU-(GlcNAc)3, were produced by co-crystallization and soaking, respectively, and the crystal structures were solved at 2.0 A resolution., The results show that 4-MeU-(GlcNAc)3 binds in subsites A-D and that, 4-MeU-(GlcNAc)2 binds in subsites B-D in the active-site cleft of RBTL., This agrees well with earlier crystallographic studies on the binding of, oligosaccharides of chitin to RBTL, which showed that (GlcNAc)3 binds to, sites B-D in RBTL and not to A-C as seen in the human and turkey egg-white, lysozymes. For both complexes the 4-MeU moiety in site D has diffuse, electron density and is flexible, as it is only bound to water molecules, and not to the protein. Since no electron density was observed in site E, the solved structures give views of nonproductive enzyme-substrate, complexes.
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<StructureSection load='1bb6' size='340' side='right'caption='[[1bb6]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1bb6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oncorhynchus_mykiss Oncorhynchus mykiss]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BB6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BB6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=UMG:METHYL-UMBELLIFERTL-N-ACETYL-CHITOTRIOSE'>UMG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bb6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bb6 OCA], [https://pdbe.org/1bb6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bb6 RCSB], [https://www.ebi.ac.uk/pdbsum/1bb6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bb6 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LYSC2_ONCMY LYSC2_ONCMY] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has antibacterial activity against the Gram positive bacterium P.citreus. Has no antibacterial activity against the Gram negative bacteria E.coli and Y.ruckeri. Does not have hemolytic activity against trout erythrocytes.<ref>PMID:15142536</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bb/1bb6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bb6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Two complexes between rainbow trout lysozyme (RBTL) and 4-methylumbelliferyl chitobioside, 4MeU-(GlcNAc)2, and chitotrioside, 4MeU-(GlcNAc)3, were produced by co-crystallization and soaking, respectively, and the crystal structures were solved at 2.0 A resolution. The results show that 4-MeU-(GlcNAc)3 binds in subsites A-D and that 4-MeU-(GlcNAc)2 binds in subsites B-D in the active-site cleft of RBTL. This agrees well with earlier crystallographic studies on the binding of oligosaccharides of chitin to RBTL, which showed that (GlcNAc)3 binds to sites B-D in RBTL and not to A-C as seen in the human and turkey egg-white lysozymes. For both complexes the 4-MeU moiety in site D has diffuse electron density and is flexible, as it is only bound to water molecules and not to the protein. Since no electron density was observed in site E, the solved structures give views of nonproductive enzyme-substrate complexes.
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==About this Structure==
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Structural studies on the binding of 4-methylumbelliferone glycosides of chitin to rainbow trout lysozyme.,Vollan VB, Hough E, Karlsen S Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):60-6. Epub 1999 Jan, 1. PMID:10089395<ref>PMID:10089395</ref>
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1BB6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Oncorhynchus_mykiss Oncorhynchus mykiss] with <scene name='pdbligand=UMG:'>UMG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Known structural/functional Site: <scene name='pdbsite=NUL:Catalytically+Active+Residues'>NUL</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BB6 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural studies on the binding of 4-methylumbelliferone glycosides of chitin to rainbow trout lysozyme., Vollan VB, Hough E, Karlsen S, Acta Crystallogr D Biol Crystallogr. 1999 Jan;55(Pt 1):60-6. Epub 1999 Jan, 1. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10089395 10089395]
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</div>
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[[Category: Lysozyme]]
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<div class="pdbe-citations 1bb6" style="background-color:#fffaf0;"></div>
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[[Category: Oncorhynchus mykiss]]
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[[Category: Single protein]]
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[[Category: Hough, E.]]
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[[Category: Karlsen, S.]]
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[[Category: Vollan, V.B.]]
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[[Category: UMG]]
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[[Category: hydrolase]]
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[[Category: n-acetyl-muramidase]]
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[[Category: umbelliferone glycosides]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:32:31 2008''
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==See Also==
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*[[Lysozyme 3D structures|Lysozyme 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Oncorhynchus mykiss]]
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[[Category: Hough E]]
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[[Category: Karlsen S]]
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[[Category: Vollan VB]]

Current revision

LYSOZYME COMPLEX WITH 4-METHYL-UMBELLIFERYL CHITOTRIOSE

PDB ID 1bb6

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