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1bxv

From Proteopedia

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[[Image:1bxv.jpg|left|200px]]<br /><applet load="1bxv" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bxv, resolution 1.8&Aring;" />
 
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'''REDUCED PLASTOCYANIN FROM SYNECHOCOCCUS SP.'''<br />
 
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==Overview==
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==REDUCED PLASTOCYANIN FROM SYNECHOCOCCUS SP.==
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The crystal structures of oxidized and reduced plastocyanins from, Synechococcus sp. PCC 7942 have been determined at 1.9 and 1.8 A, resolution, respectively, at pH 5.0. The protein consists of only 91 amino, acid residues, the smallest number known for a plastocyanin, and, apparently lacks the mostly conserved acidic patch that is believed to be, important for recognition with electron-transfer partners. The protein has, two acidic residues, Glu42 and Glu85, around Tyr83, which is thought to be, a possible conduit for electrons, but these are neutralized by Arg88 and, Lys58. Residue Arg88 interacts with Tyr83 through a pi-pi interaction in, which the guanidinium group of the former completely overlaps the aromatic, ring of the tyrosine. Reduction of the protein at pH 5.0 causes a, lengthening of one Cu-N(His) bond by 0.36 A, despite the small rms, deviation of 0.08 A calculated for the backbone atoms. Moreover, significant conformational changes of Arg88 and Lys58, along with the, movement of a water molecule adjacent to the OH group of Tyr83, were, observed on reduction; the guanidinium group of Arg88 rotates by more than, 11 degrees, and the water molecule moves by 0.42 A. The changes around the, copper site and the alterations around Tyr83 may be linked to the, reduction of the copper.
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<StructureSection load='1bxv' size='340' side='right'caption='[[1bxv]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1bxv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_elongatus_PCC_7942_=_FACHB-805 Synechococcus elongatus PCC 7942 = FACHB-805]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BXV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BXV FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bxv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bxv OCA], [https://pdbe.org/1bxv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bxv RCSB], [https://www.ebi.ac.uk/pdbsum/1bxv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bxv ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PLAS_SYNE7 PLAS_SYNE7] Participates in electron transfer between P700 and the cytochrome b6-f complex in photosystem I.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bx/1bxv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bxv ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1BXV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.] with <scene name='pdbligand=CU:'>CU</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=CUB:Cu+Binding+Site'>CUB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BXV OCA].
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*[[Plastocyanin 3D structures|Plastocyanin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure determinations of oxidized and reduced plastocyanin from the cyanobacterium Synechococcus sp. PCC 7942., Inoue T, Sugawara H, Hamanaka S, Tsukui H, Suzuki E, Kohzuma T, Kai Y, Biochemistry. 1999 May 11;38(19):6063-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10320332 10320332]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Synechococcus elongatus PCC 7942 = FACHB-805]]
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[[Category: Synechococcus sp.]]
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[[Category: Hamanaka S]]
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[[Category: Hamanaka, S.]]
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[[Category: Inoue T]]
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[[Category: Inoue, T.]]
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[[Category: Kai Y]]
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[[Category: Kai, Y.]]
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[[Category: Kohzuma T]]
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[[Category: Kohzuma, T.]]
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[[Category: Sugawara H]]
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[[Category: Sugawara, H.]]
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[[Category: Suzuki E]]
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[[Category: Suzuki, E.]]
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[[Category: Tsukui H]]
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[[Category: Tsukui, H.]]
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[[Category: CU]]
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[[Category: copper protein]]
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[[Category: electron transfer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:34:13 2008''
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Current revision

REDUCED PLASTOCYANIN FROM SYNECHOCOCCUS SP.

PDB ID 1bxv

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