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TonB

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<StructureSection load='1ihr' size='350' side='right' scene='' caption='E. coli TonB C-terminal dimer complex with Br- ions (dark red) (PDB code [[1ihr]])'>
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{{STRUCTURE_1xx3| PDB=1xx3 | SIZE=400| SCENE=TonB/Ctdtonb/1 |right|CAPTION=E. coli TonB, [[1xx3]] }}
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==Structure==
==Structure==
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The structure to the right (1XX3) shows the structure of TonB. The protein spans the periplasm <ref>PMID: 16741125</ref>, with the C-terminus of TonB spanning from residues ~150 to 239<ref name='Postle'>PMID: 21179522</ref>.
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'''TonB''' spans the periplasm <ref>PMID: 16741125</ref>, with the C-terminus of TonB spanning from residues ~150 to 239<ref name='Postle'>PMID: 21179522</ref>. For more details on the Ton system see [[Ton]].
==Function==
==Function==
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===BtuB-TonB Complex===
===BtuB-TonB Complex===
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{{STRUCTURE_2gsk | PDB=2gsk | SCENE= BtuB-TonB_Complex/Btubtonbcomplex/1 }}
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<StructureSection load='2gsk' size='350' side='right' scene='BtuB-TonB_Complex/Btubtonbcomplex/1' caption='E. coli TonB C-terminal (green) complex with vitamin B12 transporter BtuB (grey) (PDB code [[2gsk]])'>
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{{STRUCTURE_2gsk| PDB=2gsk | SIZE=400| SCENE=BtuB-TonB_Complex/Btubtonbcomplex/1 |right|CAPTION=E. coli TonB C-terminal complex with vitamin B12 transporter BtuB, [[2gsk]] }}
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As shown in the 3D structure to the right (2GSK), TonB complexes with [[BtuB]] in order to aid the transport of nutrients such as cobalamins<ref name='Cadieux'>PMID 11029413</ref> across the outer membrane by incorporating the proton-motive force into the outer membrane.<ref name='Shultis'>PMID: 16741124</ref> TonB attaches to BtuB on the periplasmic side of the 614 amino acid BtuB protein<ref name='Shultis'> PMID: 16741124</ref>.
As shown in the 3D structure to the right (2GSK), TonB complexes with [[BtuB]] in order to aid the transport of nutrients such as cobalamins<ref name='Cadieux'>PMID 11029413</ref> across the outer membrane by incorporating the proton-motive force into the outer membrane.<ref name='Shultis'>PMID: 16741124</ref> TonB attaches to BtuB on the periplasmic side of the 614 amino acid BtuB protein<ref name='Shultis'> PMID: 16741124</ref>.
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</StructureSection>
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==3D structures of TonB==
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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[[1ihr]], [[1qxx]], [[1u07]] – EcTonB C terminal – ''Escherichia coli''<br />
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[[1xx3]], [[5lw8]] - EcTonB C terminal – NMR<br />
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[[2grx]] - EcTonB + ferrichrome-iron receptor<br />
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[[2ivz]] - EcTonB + colicin-E9 peptide<br />
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[[2gsk]] - EcTonB C terminal + vitamin B12 transporter BtuB<br />
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[[2k9k]] – TonB2 C terminal – ''Listonella anguillarum''<br />
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[[6fip]] - PaTonB C terminal – ''Pseudomonas aeruginosa'' - NMR<br />
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[[6i97]] - PaTonB C terminal + TonB-dependent receptor <br />
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[[5lw8]], [[6sly]] - TonB C terminal – ''Helicobacter pylori'' - NMR<br />
== References==
== References==
<references/>
<references/>
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[[Category:Topic Page]]

Current revision

E. coli TonB C-terminal dimer complex with Br- ions (dark red) (PDB code 1ihr)

Drag the structure with the mouse to rotate

3D structures of TonB

Updated on 06-December-2020

1ihr, 1qxx, 1u07 – EcTonB C terminal – Escherichia coli
1xx3, 5lw8 - EcTonB C terminal – NMR
2grx - EcTonB + ferrichrome-iron receptor
2ivz - EcTonB + colicin-E9 peptide
2gsk - EcTonB C terminal + vitamin B12 transporter BtuB
2k9k – TonB2 C terminal – Listonella anguillarum
6fip - PaTonB C terminal – Pseudomonas aeruginosa - NMR
6i97 - PaTonB C terminal + TonB-dependent receptor
5lw8, 6sly - TonB C terminal – Helicobacter pylori - NMR

References

  1. ↑ Pawelek PD, Croteau N, Ng-Thow-Hing C, Khursigara CM, Moiseeva N, Allaire M, Coulton JW. Structure of TonB in complex with FhuA, E. coli outer membrane receptor. Science. 2006 Jun 2;312(5778):1399-402. PMID:16741125 doi:312/5778/1399
  2. ↑ 2.0 2.1 Postle K, Kastead KA, Gresock MG, Ghosh J, Swayne CD. The TonB Dimeric Crystal Structures Do Not Exist In Vivo. MBio. 2010 Dec 21;1(5). pii: e00307-10. PMID:21179522 doi:10.1128/mBio.00307-10
  3. ↑ 3.0 3.1 Kampfenkel K, Braun V. Topology of the ExbB protein in the cytoplasmic membrane of Escherichia coli. J Biol Chem. 1993 Mar 15;268(8):6050-7. PMID:8449962
  4. ↑ Wiener MC. TonB-dependent outer membrane transport: going for Baroque? Curr Opin Struct Biol. 2005 Aug;15(4):394-400. PMID:16039843 doi:10.1016/j.sbi.2005.07.001
  5. ↑ Held KG, Postle K. ExbB and ExbD do not function independently in TonB-dependent energy transduction. J Bacteriol. 2002 Sep;184(18):5170-3. PMID:12193634
  6. ↑ Cadieux N, Bradbeer C, Kadner RJ. Sequence changes in the ton box region of BtuB affect its transport activities and interaction with TonB protein. J Bacteriol. 2000 Nov;182(21):5954-61. PMID:11029413
  7. ↑ 7.0 7.1 Shultis DD, Purdy MD, Banchs CN, Wiener MC. Outer membrane active transport: structure of the BtuB:TonB complex. Science. 2006 Jun 2;312(5778):1396-9. PMID:16741124 doi:312/5778/1396

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