2x8j

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[[Image:2x8j.png|left|200px]]
 
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==Intracellular subtilisin precursor from B. clausii==
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The line below this paragraph, containing "STRUCTURE_2x8j", creates the "Structure Box" on the page.
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<StructureSection load='2x8j' size='340' side='right'caption='[[2x8j]], [[Resolution|resolution]] 1.56&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2x8j]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Alkalihalobacillus_clausii Alkalihalobacillus clausii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X8J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2X8J FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.56&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=CSX:S-OXY+CYSTEINE'>CSX</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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{{STRUCTURE_2x8j| PDB=2x8j | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2x8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2x8j OCA], [https://pdbe.org/2x8j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2x8j RCSB], [https://www.ebi.ac.uk/pdbsum/2x8j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2x8j ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/D0AB41_ALKCL D0AB41_ALKCL]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x8/2x8j_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2x8j ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The intracellular subtilisin proteases (ISPs) are the only known members of the important and ubiquitous subtilisin family that function exclusively within the cell, constituting a major component of the degradome in many Gram-positive bacteria. The first ISP structure reported herein at a spacing of 1.56 A reveals features unique among subtilisins that has enabled potential functional and physiological roles to be assigned to sequence elements exclusive to the ISPs. Unlike all other subtilisins, ISP from B. clausii is dimeric, with residues from the C terminus making a major contribution to the dimer interface by crossing over to contact the partner subunit. A short N-terminal extension binds back across the active site to provide a potential novel regulatory mechanism of intrinsic proteolytic activity: a proline residue conserved throughout the ISPs introduces a kink in the polypeptide backbone that lifts the target peptide bond out of reach of the catalytic residues.
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===INTRACELLULAR SUBTILISIN PRECURSOR FROM B. CLAUSII===
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Crystal structure of an intracellular subtilisin reveals novel structural features unique to this subtilisin family.,Vevodova J, Gamble M, Kunze G, Ariza A, Dodson E, Jones DD, Wilson KS Structure. 2010 Jun 9;18(6):744-55. PMID:20541512<ref>PMID:20541512</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20541512}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2x8j" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20541512 is the PubMed ID number.
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{{ABSTRACT_PUBMED_20541512}}
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==About this Structure==
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[[2x8j]] is a 6 chain structure of [[Subtilisin]] with sequence from [http://en.wikipedia.org/wiki/Bacillus_clausii Bacillus clausii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2X8J OCA].
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==See Also==
==See Also==
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*[[Subtilisin]]
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*[[Subtilisin 3D structures|Subtilisin 3D structures]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:020541512</ref><references group="xtra"/>
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__TOC__
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[[Category: Bacillus clausii]]
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</StructureSection>
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[[Category: Subtilisin]]
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[[Category: Alkalihalobacillus clausii]]
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[[Category: Ariza, A.]]
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[[Category: Large Structures]]
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[[Category: Dodson, E.]]
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[[Category: Ariza A]]
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[[Category: Gamble, M.]]
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[[Category: Dodson E]]
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[[Category: Jones, D D.]]
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[[Category: Gamble M]]
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[[Category: Vedodova, J.]]
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[[Category: Jones DD]]
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[[Category: Wilson, K S.]]
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[[Category: Vedodova J]]
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[[Category: Hydrolase]]
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[[Category: Wilson KS]]
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[[Category: Intracellular proteinase regulation]]
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[[Category: Serine protease]]
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Current revision

Intracellular subtilisin precursor from B. clausii

PDB ID 2x8j

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OCA

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