2kok

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[[Image:2kok.png|left|200px]]
 
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==Solution structure of an arsenate reductase (ArsC) related protein from Brucella melitensis. Seattle Structural Genomics Center for Infectious Disease target BrabA.00007.a.==
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The line below this paragraph, containing "STRUCTURE_2kok", creates the "Structure Box" on the page.
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<StructureSection load='2kok' size='340' side='right'caption='[[2kok]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2kok]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Brucella_abortus Brucella abortus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KOK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kok FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kok OCA], [https://pdbe.org/2kok PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kok RCSB], [https://www.ebi.ac.uk/pdbsum/2kok PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kok ProSAT]</span></td></tr>
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{{STRUCTURE_2kok| PDB=2kok | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q2YMA4_BRUA2 Q2YMA4_BRUA2]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ko/2kok_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kok ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Brucella melitensis is the etiological agent responsible for brucellosis. Present in the B. melitensis genome is a 116-residue protein related to arsenate reductases (Bm-YffB; BR0369). Arsenate reductases (ArsC) convert arsenate ion (H(2)AsO(4)(-)), a compound that is toxic to bacteria, to arsenite ion (AsO(2)(-)), a product that may be efficiently exported out of the cell. Consequently, Bm-YffB is a potential drug target because if arsenate reduction is the protein's major biological function then disabling the cell's ability to reduce arsenate would make these cells more sensitive to the deleterious effects of arsenate. Size-exclusion chromatography and NMR spectroscopy indicate that Bm-YffB is a monomer in solution. The solution structure of Bm-YffB (PDB entry 2kok) shows that the protein consists of two domains: a four-stranded mixed beta-sheet flanked by two alpha-helices on one side and an alpha-helical bundle. The alpha/beta domain is characteristic of the fold of thioredoxin-like proteins and the overall structure is generally similar to those of known arsenate reductases despite the marginal sequence similarity. Chemical shift perturbation studies with (15)N-labeled Bm-YffB show that the protein binds reduced glutathione at a site adjacent to a region similar to the HX(3)CX(3)R catalytic sequence motif that is important for arsenic detoxification activity in the classical arsenate-reductase family of proteins. The latter observation supports the hypothesis that the ArsC-YffB family of proteins may function as glutathione-dependent thiol reductases. However, comparison of the structure of Bm-YffB with the structures of proteins from the classical ArsC family suggest that the mechanism and possibly the function of Bm-YffB and other related proteins (ArsC-YffB) may differ from those of the ArsC family of proteins.
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===Solution structure of an arsenate reductase (ArsC) related protein from Brucella melitensis. Seattle Structural Genomics Center for Infectious Disease target BrabA.00007.a.===
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Solution structure of an arsenate reductase-related protein, YffB, from Brucella melitensis, the etiological agent responsible for brucellosis.,Buchko GW, Hewitt SN, Napuli AJ, Van Voorhis WC, Myler PJ Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Sep 1;67(Pt, 9):1129-36. Epub 2011 Aug 16. PMID:21904062<ref>PMID:21904062</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2kok" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_21904062}}, adds the Publication Abstract to the page
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*[[Arsenate reductase 3D structures|Arsenate reductase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 21904062 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21904062}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[2kok]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Brucella_abortus Brucella abortus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KOK OCA].
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==Reference==
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<ref group="xtra">PMID:021904062</ref><references group="xtra"/>
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[[Category: Brucella abortus]]
[[Category: Brucella abortus]]
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[[Category: Buchko, G W.]]
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[[Category: Large Structures]]
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[[Category: Hewitt, S N.]]
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[[Category: Buchko GW]]
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[[Category: Napuli, A J.]]
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[[Category: Hewitt SN]]
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[[Category: Voorhis, W C.Van.]]
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[[Category: Napuli AJ]]
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[[Category: Arsenate reductase]]
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[[Category: Van Voorhis WC]]
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[[Category: Brucellosis]]
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[[Category: Oxidoreductase]]
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[[Category: Seattle structural genomics center for infectious disease]]
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[[Category: Ssgcid]]
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[[Category: Structural genomic]]
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[[Category: Zoonotic]]
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Current revision

Solution structure of an arsenate reductase (ArsC) related protein from Brucella melitensis. Seattle Structural Genomics Center for Infectious Disease target BrabA.00007.a.

PDB ID 2kok

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