3q9t

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[[Image:3q9t.jpg|left|200px]]
 
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==Crystal structure analysis of formate oxidase==
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The line below this paragraph, containing "STRUCTURE_3q9t", creates the "Structure Box" on the page.
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<StructureSection load='3q9t' size='340' side='right'caption='[[3q9t]], [[Resolution|resolution]] 2.24&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3q9t]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_oryzae_RIB40 Aspergillus oryzae RIB40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q9T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3Q9T FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.24&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FAY:[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl+(2R,3S,4S)-5-(8-formyl-7-methyl-2,4-dioxo-3,4-dihydrobenzo[g]pteridin-10(2H)-yl)-2,3,4-trihydroxypentyl+dihydrogen+diphosphate'>FAY</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
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{{STRUCTURE_3q9t| PDB=3q9t | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3q9t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3q9t OCA], [https://pdbe.org/3q9t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3q9t RCSB], [https://www.ebi.ac.uk/pdbsum/3q9t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3q9t ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q2UD26_ASPOR Q2UD26_ASPOR]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Formate oxidase of Aspergillus oryzae RIB40 contains an 8-replaced FAD with molecular mass of 799 as cofactor. The (1)H-NMR spectrum of the cofactor fraction obtained from the enzyme indicated that the 8-replaced FAD in the fraction was 8-formyl-FAD, present in open form and hemiacetal form. The oxidation-reduction potentials of the open and hemiacetal forms were estimated by cyclic voltammetry to be -47 and -177 mV vs. Normal Hydrogen Electrode respectively. The structure of the enzyme was constructed using diffraction data to 2.24 A resolution collected from a crystal of the enzyme. His(511) and Arg(554) were situated close to the pyrimidine part of the isoalloxazine ring of 8-formyl-FAD in open form. The enzyme had 8-formyl-FAD, the oxidation potential of which was approximately 160 mV more positive than that of FAD, and the His-Arg pair at the catalytic site, unlike the other enzymes belonging to the glucose-methanol-choline oxidoreductase family.
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===Crystal structure analysis of formate oxidase===
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Formate Oxidase, an Enzyme of the Glucose-Methanol-Choline Oxidoreductase Family, Has a His-Arg Pair and 8-Formyl-FAD at the Catalytic Site.,Doubayashi D, Ootake T, Maeda Y, Oki M, Tokunaga Y, Sakurai A, Nagaosa Y, Mikami B, Uchida H Biosci Biotechnol Biochem. 2011 Sep 7. PMID:21897046<ref>PMID:21897046</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3q9t" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 21897046 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21897046}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Aspergillus oryzae RIB40]]
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[[3q9t]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3Q9T OCA].
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[[Category: Large Structures]]
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[[Category: Doubayashi D]]
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==Reference==
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[[Category: Maeda Y]]
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<ref group="xtra">PMID:021897046</ref><ref group="xtra">PMID:020823527</ref><references group="xtra"/>
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[[Category: Mikami B]]
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[[Category: Aspergillus oryzae]]
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[[Category: Nagaosa Y]]
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[[Category: Doubayashi, D.]]
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[[Category: Oki M]]
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[[Category: Maeda, Y.]]
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[[Category: Ootake T]]
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[[Category: Mikami, B.]]
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[[Category: Sakurai A]]
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[[Category: Nagaosa, Y.]]
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[[Category: Tokunaga Y]]
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[[Category: Oki, M.]]
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[[Category: Uchida H]]
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[[Category: Ootake, T.]]
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[[Category: Sakurai, A.]]
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[[Category: Tokunaga, Y.]]
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[[Category: Uchida, H.]]
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[[Category: 8-formyl-fad]]
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[[Category: Formate oxidase]]
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[[Category: Glucose-methanol-choline oxidoreductase family]]
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[[Category: Oxidoreductase]]
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Current revision

Crystal structure analysis of formate oxidase

PDB ID 3q9t

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