1gk8

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[[Image:1gk8.gif|left|200px]]<br /><applet load="1gk8" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gk8, resolution 1.4&Aring;" />
 
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'''RUBISCO FROM CHLAMYDOMONAS REINHARDTII'''<br />
 
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==Overview==
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==Rubisco from Chlamydomonas reinhardtii==
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The crystal structure of Rubisco (ribulose 1,5-bisphosphate, carboxylase/oxygenase) from the unicellular green alga Chlamydomonas, reinhardtii has been determined to 1.4 A resolution. Overall, the, structure shows high similarity to the previously determined structures of, L8S8 Rubisco enzymes. The largest difference is found in the loop between, beta strands A and B of the small subunit (betaA-betaB loop), which is, longer by six amino acid residues than the corresponding region in Rubisco, from Spinacia. Mutations of residues in the betaA-betaB loop have been, shown to affect holoenzyme stability and catalytic properties. The, information contained in the Chlamydomonas structure enables a more, reliable analysis of the effect of these mutations. No electron density, was observed for the last 13 residues of the small subunit, which are, assumed to be disordered in the crystal. Because of the high resolution of, the data, some posttranslational modifications are unambiguously apparent, in the structure. These include cysteine and N-terminal methylations and, proline 4-hydroxylations.
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<StructureSection load='1gk8' size='340' side='right'caption='[[1gk8]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1gk8]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GK8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAP:2-CARBOXYARABINITOL-1,5-DIPHOSPHATE'>CAP</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=HYP:4-HYDROXYPROLINE'>HYP</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MME:N-METHYL+METHIONINE'>MME</scene>, <scene name='pdbligand=SMC:S-METHYLCYSTEINE'>SMC</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gk8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gk8 OCA], [https://pdbe.org/1gk8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gk8 RCSB], [https://www.ebi.ac.uk/pdbsum/1gk8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gk8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RBL_CHLRE RBL_CHLRE] RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.[HAMAP-Rule:MF_01338]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gk/1gk8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gk8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of Rubisco (ribulose 1,5-bisphosphate carboxylase/oxygenase) from the unicellular green alga Chlamydomonas reinhardtii has been determined to 1.4 A resolution. Overall, the structure shows high similarity to the previously determined structures of L8S8 Rubisco enzymes. The largest difference is found in the loop between beta strands A and B of the small subunit (betaA-betaB loop), which is longer by six amino acid residues than the corresponding region in Rubisco from Spinacia. Mutations of residues in the betaA-betaB loop have been shown to affect holoenzyme stability and catalytic properties. The information contained in the Chlamydomonas structure enables a more reliable analysis of the effect of these mutations. No electron density was observed for the last 13 residues of the small subunit, which are assumed to be disordered in the crystal. Because of the high resolution of the data, some posttranslational modifications are unambiguously apparent in the structure. These include cysteine and N-terminal methylations and proline 4-hydroxylations.
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==About this Structure==
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First crystal structure of Rubisco from a green alga, Chlamydomonas reinhardtii.,Taylor TC, Backlund A, Bjorhall K, Spreitzer RJ, Andersson I J Biol Chem. 2001 Dec 21;276(51):48159-64. Epub 2001 Oct 18. PMID:11641402<ref>PMID:11641402</ref>
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1GK8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=CAP:'>CAP</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribulose-bisphosphate_carboxylase Ribulose-bisphosphate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.39 4.1.1.39] Known structural/functional Site: <scene name='pdbsite=MGA:Active+Site+Residues+Coordinating+Mg'>MGA</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GK8 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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First crystal structure of Rubisco from a green alga, Chlamydomonas reinhardtii., Taylor TC, Backlund A, Bjorhall K, Spreitzer RJ, Andersson I, J Biol Chem. 2001 Dec 21;276(51):48159-64. Epub 2001 Oct 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11641402 11641402]
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</div>
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[[Category: Chlamydomonas reinhardtii]]
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<div class="pdbe-citations 1gk8" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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[[Category: Ribulose-bisphosphate carboxylase]]
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[[Category: Andersson, I.]]
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[[Category: Spreitzer, R.J.]]
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[[Category: Taylor, T.C.]]
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[[Category: CAP]]
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[[Category: EDO]]
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[[Category: MG]]
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[[Category: lyase]]
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[[Category: photosynthesis]]
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[[Category: rubisco]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:40:36 2008''
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==See Also==
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*[[RuBisCO 3D structures|RuBisCO 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Chlamydomonas reinhardtii]]
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[[Category: Large Structures]]
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[[Category: Taylor TC]]

Current revision

Rubisco from Chlamydomonas reinhardtii

PDB ID 1gk8

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