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3aml

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[[Image:3aml.jpg|left|200px]]
 
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==Structure of the Starch Branching Enzyme I (BEI) from Oryza sativa L==
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The line below this paragraph, containing "STRUCTURE_3aml", creates the "Structure Box" on the page.
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<StructureSection load='3aml' size='340' side='right'caption='[[3aml]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3aml]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3AML FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SIN:SUCCINIC+ACID'>SIN</scene></td></tr>
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{{STRUCTURE_3aml| PDB=3aml | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3aml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3aml OCA], [https://pdbe.org/3aml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3aml RCSB], [https://www.ebi.ac.uk/pdbsum/3aml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3aml ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GLGB_ORYSJ GLGB_ORYSJ] Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Starch-branching enzyme catalyzes the cleavage of alpha-1, 4-linkages and the subsequent transfer of alpha-1,4 glucan to form an alpha-1,6 branch point in amylopectin. Sequence analysis of the rice-branching enzyme I (BEI) indicated a modular structure in which the central alpha-amylase domain is flanked on each side by the N-terminal carbohydrate-binding module 48 and the alpha-amylase C-domain. We determined the crystal structure of BEI at a resolution of 1.9 A by molecular replacement using the Escherichia coli glycogen BE as a search model. Despite three modular structures, BEI is roughly ellipsoidal in shape with two globular domains that form a prominent groove which is proposed to serve as the alpha-polyglucan-binding site. Amino acid residues Asp344 and Glu399, which are postulated to play an essential role in catalysis as a nucleophile and a general acid/base, respectively, are located at a central cleft in the groove. Moreover, structural comparison revealed that in BEI, extended loop structures cause a narrowing of the substrate-binding site, whereas shortened loop structures make a larger space at the corresponding subsite in the Klebsiella pneumoniae pullulanase. This structural difference might be attributed to distinct catalytic reactions, transglycosylation and hydrolysis, respectively, by BEI and pullulanase.
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===Structure of the Starch Branching Enzyme I (BEI) from Oryza sativa L===
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Crystal structure of the branching enzyme I (BEI) from Oryza sativa L with implications for catalysis and substrate binding.,Noguchi J, Chaen K, Vu NT, Akasaka T, Shimada H, Nakashima T, Nishi A, Satoh H, Omori T, Kakuta Y, Kimura M Glycobiology. 2011 Aug;21(8):1108-16. Epub 2011 Apr 14. PMID:21493662<ref>PMID:21493662</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_21493662}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 3aml" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 21493662 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_21493662}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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[[3aml]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oryza_sativa_japonica_group Oryza sativa japonica group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3AML OCA].
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[[Category: Oryza sativa Japonica Group]]
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[[Category: Chaen K]]
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==Reference==
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[[Category: Kakuta Y]]
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<ref group="xtra">PMID:021493662</ref><references group="xtra"/>
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[[Category: Kimura M]]
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[[Category: Oryza sativa japonica group]]
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[[Category: Noguchi J]]
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[[Category: Chaen, K.]]
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[[Category: Vu N]]
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[[Category: Kakuta, Y.]]
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[[Category: Kimura, M.]]
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[[Category: Noguchi, J.]]
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[[Category: Vu, N.]]
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[[Category: Starch-branching]]
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[[Category: Transferase]]
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Current revision

Structure of the Starch Branching Enzyme I (BEI) from Oryza sativa L

PDB ID 3aml

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