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| - | [[Image:1gwl.jpg|left|200px]]<br /><applet load="1gwl" size="350" color="white" frame="true" align="right" spinBox="true" | |
| - | caption="1gwl, resolution 1.51Å" /> | |
| - | '''CARBOHYDRATE BINDING MODULE FAMILY29 COMPLEXED WITH MANNOHEXAOSE'''<br /> | |
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| - | ==Overview== | + | ==Carbohydrate binding module family29 complexed with mannohexaose== |
| - | Carbohydrate-protein recognition is central to many biological processes., Enzymes that act on polysaccharide substrates frequently contain, noncatalytic domains, "carbohydrate-binding modules" (CBMs), that target, the enzyme to the appropriate substrate. CBMs that recognize specific, plant structural polysaccharides are often able to accommodate both the, variable backbone and the side-chain decorations of heterogeneous ligands., "CBM29" modules, derived from a noncatalytic component of the Piromyces, equi cellulase/hemicellulase complex, provide an example of this selective, yet flexible recognition. They discriminate strongly against some, polysaccharides while remaining relatively promiscuous toward both, beta-1,4-linked manno- and cello-oligosaccharides. This feature may, reflect preferential, but flexible, targeting toward glucomannans in the, plant cell wall. The three-dimensional structure of CBM29-2 and its, complexes with cello- and mannohexaose reveal a beta-jelly-roll topology, with an extended binding groove on the concave surface. The orientation of, the aromatic residues complements the conformation of the target sugar, polymer while accommodation of both manno- and gluco-configured oligo- and, polysaccharides is conferred by virtue of the plasticity of the direct, interactions from their axial and equatorial 2-hydroxyls, respectively., Such flexible ligand recognition targets the anaerobic fungal complex to a, range of different components in the plant cell wall and thus plays a, pivotal role in the highly efficient degradation of this composite, structure by the microbial eukaryote.
| + | <StructureSection load='1gwl' size='340' side='right'caption='[[1gwl]], [[Resolution|resolution]] 1.51Å' scene=''> |
| - | | + | == Structural highlights == |
| - | ==About this Structure== | + | <table><tr><td colspan='2'>[[1gwl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Piromyces_sp._'equi' Piromyces sp. 'equi']. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GWL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GWL FirstGlance]. <br> |
| - | 1GWL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Piromyces_equi Piromyces equi]. Known structural/functional Site: <scene name='pdbsite=BM1:Bma+Binding+Site+For+Chain+A+Residue+A1148'>BM1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GWL OCA].
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.51Å</td></tr> |
| - | | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene></td></tr> |
| - | ==Reference== | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gwl OCA], [https://pdbe.org/1gwl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gwl RCSB], [https://www.ebi.ac.uk/pdbsum/1gwl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gwl ProSAT]</span></td></tr> |
| - | Promiscuity in ligand-binding: The three-dimensional structure of a Piromyces carbohydrate-binding module, CBM29-2, in complex with cello- and mannohexaose., Charnock SJ, Bolam DN, Nurizzo D, Szabo L, McKie VA, Gilbert HJ, Davies GJ, Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14077-82. Epub 2002 Oct 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12391332 12391332]
| + | </table> |
| - | [[Category: Piromyces equi]] | + | == Function == |
| - | [[Category: Single protein]]
| + | [https://www.uniprot.org/uniprot/Q9C171_PIREQ Q9C171_PIREQ] |
| - | [[Category: Charnock, S.J.]] | + | __TOC__ |
| - | [[Category: Davies, G.J.]] | + | </StructureSection> |
| - | [[Category: Nurizzo, D.]] | + | [[Category: Large Structures]] |
| - | [[Category: carbohydrate binding domain]] | + | [[Category: Piromyces sp. 'equi']] |
| - | [[Category: cellohexaose]] | + | [[Category: Charnock SJ]] |
| - | [[Category: cellulosome]] | + | [[Category: Davies GJ]] |
| - | [[Category: glucomannan]] | + | [[Category: Nurizzo D]] |
| - | [[Category: mannohexaose]] | + | |
| - | | + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:43:30 2008''
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