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1gxz

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[[Image:1gxz.gif|left|200px]]<br /><applet load="1gxz" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1gxz, resolution 2.1&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE EUCARYOTIC MONO-ADP-RIBOSYLTRANSFERASE ART2.2; CRYSTAL FORM B (P212121)'''<br />
 
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==Overview==
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==crystal structure of the eukaryotic mono-ADP-ribosyltransferase ART2.2; Crystal form B (P212121)==
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The mammalian extracellular ADP-ribosyl transferases ART1 through ART5 are, sequence-related to each other. Among them ART2 is involved in immuno, regulation. The variant ART2.2 was expressed in the periplasm of, Escherichia coli and crystallized. Its structure was determined by X-ray, diffraction at 1.7A resolution in one crystal form and at slightly lower, resolutions in two others. The active center was indicated by a ligated, nicotinamide analogue, which also revealed a small induced-fit. The, centerpiece of the chainfold of ART2.2 agrees with those of all bacterial, ADP-ribosyl transferases. This correspondence and the nicotinamide, position were used to model the binding structure of the whole substrate, NAD(+) at ART2.2. Two of the bacterial enzymes are structurally more, closely related to ART2.2 while the others are more closely related to the, eukaryotic poly(ADP-ribosyl)polymerase. This splits the ADP-ribosyl, transferases into two distinct subfamilies. A special feature of ART2.2 is, its long N-terminal extension and two disulfide bridges that are far away, from the active center. They stabilize the protein against denaturation, and presumably also against shearing forces parallel with the membrane, where ART2.2 is anchored.
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<StructureSection load='1gxz' size='340' side='right'caption='[[1gxz]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1gxz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GXZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GXZ FirstGlance]. <br>
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1GXZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=BRT:'>BRT</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/NAD(P)(+)--protein-arginine_ADP-ribosyltransferase NAD(P)(+)--protein-arginine ADP-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.31 2.4.2.31] Known structural/functional Site: <scene name='pdbsite=AC1:Brt+Binding+Site+For+Chain+B'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GXZ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BRT:5-BROMONICOTINAMIDE'>BRT</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gxz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gxz OCA], [https://pdbe.org/1gxz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gxz RCSB], [https://www.ebi.ac.uk/pdbsum/1gxz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gxz ProSAT]</span></td></tr>
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Structure of the ecto-ADP-ribosyl transferase ART2.2 from rat., Mueller-Dieckmann C, Ritter H, Haag F, Koch-Nolte F, Schulz GE, J Mol Biol. 2002 Sep 27;322(4):687-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12270706 12270706]
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</table>
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[[Category: NAD(P)(+)--protein-arginine ADP-ribosyltransferase]]
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== Function ==
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[https://www.uniprot.org/uniprot/NAR2B_RAT NAR2B_RAT] Has both NAD(+) glycohydrolase and ADP-ribosyltransferase activity (to a lesser extent).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gx/1gxz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gxz ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Mueller-Dieckmann C]]
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[[Category: Mueller-Dieckmann, C.]]
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[[Category: Schulz GE]]
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[[Category: Schulz, G.E.]]
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[[Category: BRT]]
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[[Category: adp-ribosyltransferase]]
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[[Category: immuno-regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:43:58 2008''
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Current revision

crystal structure of the eukaryotic mono-ADP-ribosyltransferase ART2.2; Crystal form B (P212121)

PDB ID 1gxz

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