2wx5

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[[Image:2wx5.png|left|200px]]
 
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==Hexa-coordination of a bacteriochlorophyll cofactor in the Rhodobacter sphaeroides reaction centre==
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The line below this paragraph, containing "STRUCTURE_2wx5", creates the "Structure Box" on the page.
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<StructureSection load='2wx5' size='340' side='right'caption='[[2wx5]], [[Resolution|resolution]] 2.63&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2wx5]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides Cereibacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WX5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WX5 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.63&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SPN:SPEROIDENONE'>SPN</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene></td></tr>
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{{STRUCTURE_2wx5| PDB=2wx5 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wx5 OCA], [https://pdbe.org/2wx5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wx5 RCSB], [https://www.ebi.ac.uk/pdbsum/2wx5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wx5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RCEH_CERS4 RCEH_CERS4] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wx/2wx5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wx5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structural and functional consequences of changing the coordination state of one of the bacteriochlorophyll (BChl) cofactors in the purple bacterial reaction center have been explored. A combination of steady state spectroscopy and X-ray crystallography was used to demonstrate that mutagenesis of residue 181 of the L-polypeptide from Phe to Arg (FL181R) causes the BChl at the accessory (BB) position on the so-called inactive cofactor branch to become hexa-coordinated, with no significant changes to the structure of the surrounding protein. This change was accompanied by the appearance of a distinctive absorbance band at 631 nm in the room temperature absorbance spectrum. The ligand donor was not the Arg side chain but rather an intervening water molecule, and contrary to expectations the Mg of BB did not adopt a more in-plane geometry in response to hexa-coordination. The mutation caused a disturbance to the detailed conformation of the BChl macrocycle that manifested in a number of subtle changes to the resonance Raman spectrum. Hexa-coordination of BB produced a small increase in the lifetime of the excited electronic state of the primary donor bacteriochlorophylls (P*), indicating some disturbance to light-driven energy and/or electron transfer events on the time-scale of a few picoseconds after light excitation. The BB bacteriochlorophyll returned to a penta-coordinated state in a double mutant where the FL181R mutation was combined with removal of the native axial ligand through mutation of His M182 to Leu. Experimental evidence for hexa-coordinated bacteriochlorophylls in the literature on antenna proteins is considered, and possible reasons why hexa-coordinated bacteriochlorophylls and chlorophylls appear to be avoided in photosynthetic proteins are discussed.
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===HEXA-COORDINATION OF A BACTERIOCHLOROPHYLL COFACTOR IN THE RHODOBACTER SPHAEROIDES REACTION CENTRE===
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Structural and spectroscopic consequences of hexa-coordination of a bacteriochlorophyll cofactor in the Rhodobacter sphaeroides reaction center.,Frolov D, Marsh M, Crouch LI, Fyfe PK, Robert B, van Grondelle R, Hadfield A, Jones MR Biochemistry. 2010 Jan 29. PMID:20112981<ref>PMID:20112981</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_20112981}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2wx5" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 20112981 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_20112981}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Cereibacter sphaeroides]]
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[[2wx5]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WX5 OCA].
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[[Category: Large Structures]]
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[[Category: Crouch LI]]
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==Reference==
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[[Category: Frolov D]]
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<ref group="xtra">PMID:020112981</ref><references group="xtra"/>
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[[Category: Fyfe PK]]
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[[Category: Rhodobacter sphaeroides]]
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[[Category: Hadfield AT]]
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[[Category: Crouch, L I.]]
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[[Category: Jones MR]]
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[[Category: Frolov, D.]]
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[[Category: Marsh M]]
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[[Category: Fyfe, P K.]]
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[[Category: Robert B]]
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[[Category: Hadfield, A T.]]
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[[Category: Van Grondelle R]]
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[[Category: Jones, M R.]]
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[[Category: Marsh, M.]]
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[[Category: Robert, B.]]
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[[Category: Vangrondelle, R.]]
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[[Category: Bacteriochlorophyll]]
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[[Category: Chlorophyll]]
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[[Category: Chromophore]]
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[[Category: Electron transport]]
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[[Category: Iron]]
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[[Category: Magnesium]]
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[[Category: Membrane]]
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[[Category: Membrane protein]]
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[[Category: Metal-binding]]
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[[Category: Photosynthesis]]
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[[Category: Photosynthetic reaction center]]
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[[Category: Reaction center]]
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[[Category: Transmembrane]]
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[[Category: Transport]]
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Current revision

Hexa-coordination of a bacteriochlorophyll cofactor in the Rhodobacter sphaeroides reaction centre

PDB ID 2wx5

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