1hh7

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[[Image:1hh7.jpg|left|200px]]<br /><applet load="1hh7" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1hh7, resolution 1.40&Aring;" />
 
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'''REFINED CRYSTAL STRUCTURE OF CYTOCHROME C2 FROM RHODOPSEUDOMONAS PALUSTRIS AT 1.4 ANGSTROM RESOLUTION'''<br />
 
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==Overview==
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==REFINED CRYSTAL STRUCTURE OF CYTOCHROME C2 FROM RHODOPSEUDOMONAS PALUSTRIS AT 1.4 ANGSTROM RESOLUTION==
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Cytochrome c(2) from Rhodopseudomonas palustris has been crystallized in, two different crystal forms: a monoclinic form I at pH 4.4 from both, reduced and oxidized protein solution and a trigonal form II at pH 9.0, from reduced protein solution. Complete 1. 7 and 1.4 A resolution data, sets were collected from the oxidized form I and from the form II, respectively. The preliminary structures show an important change in the, iron coordination environment in the trigonal form obtained at basic pH, arising from the substitution of the Met ligand by an ammonia molecule.
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<StructureSection load='1hh7' size='340' side='right'caption='[[1hh7]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1hh7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodopseudomonas_palustris Rhodopseudomonas palustris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HH7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HH7 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=NH3:AMMONIA'>NH3</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hh7 OCA], [https://pdbe.org/1hh7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hh7 RCSB], [https://www.ebi.ac.uk/pdbsum/1hh7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hh7 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CYC22_RHOPA CYC22_RHOPA] Cytochrome c2 is found mainly in purple, non-sulfur, photosynthetic bacteria where it functions as the electron donor to the oxidized bacteriochlorophyll in the photophosphorylation pathway. However, it may also have a role in the respiratory chain and is found in some non-photosynthetic bacteria.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hh/1hh7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hh7 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Cytochrome c(2) from Rhodopseudomonas palustris has been crystallized in two different crystal forms: a monoclinic form I at pH 4.4 from both reduced and oxidized protein solution and a trigonal form II at pH 9.0 from reduced protein solution. Complete 1. 7 and 1.4 A resolution data sets were collected from the oxidized form I and from the form II, respectively. The preliminary structures show an important change in the iron coordination environment in the trigonal form obtained at basic pH arising from the substitution of the Met ligand by an ammonia molecule.
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==About this Structure==
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Crystallization and preliminary X-ray analysis of two pH-dependent forms of cytochrome c2 from Rhodopseudomonas palustris.,Garau G, Geremia S, Randaccio L, Vaccari L, Viezzoli MS Acta Crystallogr D Biol Crystallogr. 2000 Dec;56(Pt 12):1699-701. PMID:11092951<ref>PMID:11092951</ref>
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1HH7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhodopseudomonas_palustris Rhodopseudomonas palustris] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=HEC:'>HEC</scene> and <scene name='pdbligand=NH3:'>NH3</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=HEC:Hec+Binding+Site+For+Chain+A'>HEC</scene>, <scene name='pdbsite=NH3:Nh3+Binding+Site+For+Chain+A'>NH3</scene>, <scene name='pdbsite=SO1:So4+Binding+Site+For+Residue+A300'>SO1</scene> and <scene name='pdbsite=SO2:So4+Binding+Site+For+Residue+A301'>SO2</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HH7 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystallization and preliminary X-ray analysis of two pH-dependent forms of cytochrome c2 from Rhodopseudomonas palustris., Garau G, Geremia S, Randaccio L, Vaccari L, Viezzoli MS, Acta Crystallogr D Biol Crystallogr. 2000 Dec;56(Pt 12):1699-701. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11092951 11092951]
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</div>
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[[Category: Rhodopseudomonas palustris]]
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<div class="pdbe-citations 1hh7" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Garau, G.]]
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[[Category: Geremia, S.]]
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[[Category: HEC]]
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[[Category: NH3]]
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[[Category: SO4]]
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[[Category: electron carrier]]
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[[Category: electron transport]]
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[[Category: heme protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:49:13 2008''
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==See Also==
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*[[Cytochrome C 3D structures|Cytochrome C 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Rhodopseudomonas palustris]]
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[[Category: Garau G]]
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[[Category: Geremia S]]

Current revision

REFINED CRYSTAL STRUCTURE OF CYTOCHROME C2 FROM RHODOPSEUDOMONAS PALUSTRIS AT 1.4 ANGSTROM RESOLUTION

PDB ID 1hh7

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