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1hjk

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[[Image:1hjk.gif|left|200px]]<br /><applet load="1hjk" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="1hjk, resolution 2.3&Aring;" />
 
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'''ALKALINE PHOSPHATASE MUTANT H331Q'''<br />
 
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==Overview==
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==ALKALINE PHOSPHATASE MUTANT H331Q==
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Using a mutant version of E. coli alkaline phosphatase, we succeeded in, trapping and determining the structure of the phospho-enzyme intermediate., The X-ray structure also revealed the catalytic water molecule, bound to, one of the active site zinc ions, positioned ideally for the apical attack, necessary for the hydrolysis of the intermediate.
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<StructureSection load='1hjk' size='340' side='right'caption='[[1hjk]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1hjk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HJK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjk OCA], [https://pdbe.org/1hjk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hjk RCSB], [https://www.ebi.ac.uk/pdbsum/1hjk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hjk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PPB_ECOLI PPB_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/1hjk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hjk ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1HJK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] Known structural/functional Sites: <scene name='pdbsite=CTA:Catalytically+Active+Site+On+A+Subunit'>CTA</scene> and <scene name='pdbsite=CTB:Catalytically+Active+Site+On+B+Subunit'>CTB</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJK OCA].
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*[[Alkaline phosphatase 3D structures|Alkaline phosphatase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Trapping and visualization of a covalent enzyme-phosphate intermediate., Murphy JE, Stec B, Ma L, Kantrowitz ER, Nat Struct Biol. 1997 Aug;4(8):618-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9253408 9253408]
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[[Category: Alkaline phosphatase]]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Kantrowitz, E.R.]]
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[[Category: Kantrowitz ER]]
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[[Category: Ma, L.]]
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[[Category: Ma L]]
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[[Category: Murphy, J.E.]]
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[[Category: Murphy JE]]
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[[Category: Stec, B.]]
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[[Category: Stec B]]
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[[Category: MG]]
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[[Category: SO4]]
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[[Category: ZN]]
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[[Category: alcohol acceptor)]]
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[[Category: alkaline phosphatase]]
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[[Category: hydrolase]]
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[[Category: phosphoric monoester]]
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[[Category: transferase(phospho]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 09:49:36 2008''
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Current revision

ALKALINE PHOSPHATASE MUTANT H331Q

PDB ID 1hjk

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